Galey F D, Mendez L E, Whitehead W E, Holstege D M, Plumlee K H, Johnson B
California Veterinary Diagnostic Laboratory System, Davis 95617.
J Vet Diagn Invest. 1993 Oct;5(4):603-8. doi: 10.1177/104063879300500416.
The classical mouse uterine bioassay was evaluated and adapted for routine diagnostic use in response to requests for evaluation of forages suspected of being estrogenic. Forages were extracted in acetone or 10% ethanol in acetone (v/v). Extracts were mixed with ground corn-based mouse feed. Immature female mice (n = 3/group) were fed a total of 100 g of the ground feed for 5 days. Body weights were monitored before and after the trial. After 6 days, the mice were euthanized and uterine weights were determined. Mean uterine weights were compared using 1-way analysis of variance with preselected contrasts for individual means. Selected uteruses were fixed in 10% neutral buffered formalin for histologic examination. Control feeds, diethylstilbestrol (DES), estradiol, coumestrol, feeds with no reported estrogenic properties, and a feed that caused hyperestrogenism in cattle were tested. Moderate levels of estrogenic compounds resulted in dose-responsive uterine enlargements (10-270 ppm coumestrol over 5 days). Extremely high levels of estrogen frequently resulted in feed refusal and lack of uterine enlargement (10 ppm DES, 100 ppm estradiol). Diagnostically significant estrogenic activity was recovered from the feed known to have been estrogenic in cattle. The classical mouse uterine bioassay was relatively inexpensive, quick, repeatable, and capable of detecting clinically relevant coumestrol levels in hay.
应评估疑似具有雌激素活性的草料的请求，对经典的小鼠子宫生物测定法进行了评估并调整，以用于常规诊断。草料用丙酮或10%乙醇（体积比）的丙酮溶液进行提取。提取物与磨碎的玉米基小鼠饲料混合。将未成熟雌性小鼠（每组n = 3）喂食总共100克磨碎的饲料，持续5天。在试验前后监测体重。6天后，对小鼠实施安乐死并测定子宫重量。使用单因素方差分析及对各个平均值的预选对比来比较平均子宫重量。选取的子宫用10%中性缓冲福尔马林固定，用于组织学检查。对对照饲料、己烯雌酚（DES）、雌二醇、香豆雌酚、无雌激素活性报道的饲料以及一种导致牛雌激素过多症的饲料进行了测试。中等水平的雌激素化合物导致子宫呈剂量反应性增大（5天内香豆雌酚含量为10 - 270 ppm）。极高水平的雌激素常常导致拒食且子宫不增大（10 ppm DES、100 ppm雌二醇）。从已知对牛具有雌激素活性的饲料中检测到了具有诊断意义的雌激素活性。经典的小鼠子宫生物测定法相对便宜、快速、可重复，并且能够检测干草中临床相关的香豆雌酚水平。
