Maccario R, Chirico G, Mingrat G, Aricò M, Lanfranchi A, Montagna D, Moretta A, Rondini G
Department of Pediatrics, University of Pavia, Italy.
Biol Neonate. 1993;64(6):346-53. doi: 10.1159/000244010.
Expression of CD45R0 antigen has been evaluated on the surface of T lymphocyte subsets obtained from cord blood and on peripheral blood lymphocytes (PBL) from both term and preterm neonates. In some experiments, expression of the same antigen has also been evaluated after in vitro activation of cord blood lymphocytes (CBL) with phytohemagglutinin (PHA) or in mixed lymphocyte culture. The CD45R0 molecule was found to be present on a significantly lower percentage of CD3+, CD4+ and CD8+ CBL as compared with the same subsets evaluated in adult individuals. Moreover, at variance with adult PBL, the great majority of CBL displayed a low level of fluorescence when stained with UCHL1 (CD45R0-specific monoclonal antibody), and both PHA and allogeneic stimuli were able to strongly increase the expression of the CD45R0 molecule on CBL. The percentage of CD45R0+ PBL obtained from both term and preterm neonates on the 1st day of life was comparable to that of CBL, however the expression of this molecule increases remarkably within a few days in the majority of term neonates, while it seems to take more time for the antigen to be fully expressed in preterm infants.
已对从脐血获得的T淋巴细胞亚群表面以及足月和早产新生儿的外周血淋巴细胞（PBL）表面的CD45R0抗原表达进行了评估。在一些实验中，还用植物血凝素（PHA）体外激活脐血淋巴细胞（CBL）或在混合淋巴细胞培养后评估了相同抗原的表达。与在成人个体中评估的相同亚群相比，发现CD45R0分子在CD3 +、CD4 +和CD8 + CBL上的存在百分比显著更低。此外，与成人PBL不同，当用UCHL1（CD45R0特异性单克隆抗体）染色时，绝大多数CBL显示出低水平的荧光，并且PHA和同种异体刺激都能够强烈增加CBL上CD45R0分子的表达。从足月和早产新生儿出生第一天获得的CD45R0 + PBL的百分比与CBL相当，然而，在大多数足月新生儿中，该分子的表达在几天内显著增加，而抗原在早产儿中似乎需要更长时间才能完全表达。
