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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Grote H, Ioannou I, Voigt J, Sekeris C E

Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.

Int J Biochem. 1993 Nov;25(11):1593-9. doi: 10.1016/0020-711x(93)90517-i.

The cytoplasmic glucocorticoid receptor of rat liver cells is in part recovered in the plasma membrane fraction. 2. After in vivo administration of [3H]dexamethasone, 0.35% of the radioactivity recovered is bound on plasma membranes. 3. Dexamethasone also binds in vitro specifically to plasma membranes. Expressed as fmol/mg protein, binding of dexamethasone to plasma membranes is comparable to binding to the soluble cytoplasmic fraction (cytosol). 4. Using polyclonal antibody to the glucocorticoid receptor and the indirect immunofluorescence technic, an intense decoration of the plasma membranes is observed, denoting a high concentration of glucocorticoid receptor on plasma membranes. 5. The localization of the receptor on plasma membranes could be of potential importance for its interaction with agents (mitogens, growth factors) initially acting on the cell membrane, regulating subsequent cell proliferation and growth at the level of the cell nucleus.

大鼠肝细胞的细胞质糖皮质激素受体部分存在于质膜组分中。2. 体内给予[3H]地塞米松后，回收的放射性中有0.35%与质膜结合。3. 地塞米松在体外也能特异性地与质膜结合。以fmol/mg蛋白质表示，地塞米松与质膜的结合与与可溶性细胞质组分（胞质溶胶）的结合相当。4. 使用针对糖皮质激素受体的多克隆抗体和间接免疫荧光技术，可观察到质膜有强烈的荧光标记，表明质膜上有高浓度的糖皮质激素受体。5. 受体在质膜上的定位对于其与最初作用于细胞膜的因子（有丝分裂原、生长因子）相互作用可能具有潜在重要性，这些因子可在细胞核水平调节随后的细胞增殖和生长。

Grote H, Ioannou I, Voigt J, Sekeris C E

Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.

Int J Biochem. 1993 Nov;25(11):1593-9. doi: 10.1016/0020-711x(93)90517-i.

The cytoplasmic glucocorticoid receptor of rat liver cells is in part recovered in the plasma membrane fraction. 2. After in vivo administration of [3H]dexamethasone, 0.35% of the radioactivity recovered is bound on plasma membranes. 3. Dexamethasone also binds in vitro specifically to plasma membranes. Expressed as fmol/mg protein, binding of dexamethasone to plasma membranes is comparable to binding to the soluble cytoplasmic fraction (cytosol). 4. Using polyclonal antibody to the glucocorticoid receptor and the indirect immunofluorescence technic, an intense decoration of the plasma membranes is observed, denoting a high concentration of glucocorticoid receptor on plasma membranes. 5. The localization of the receptor on plasma membranes could be of potential importance for its interaction with agents (mitogens, growth factors) initially acting on the cell membrane, regulating subsequent cell proliferation and growth at the level of the cell nucleus.

大鼠肝细胞的细胞质糖皮质激素受体部分存在于质膜组分中。2. 体内给予[3H]地塞米松后，回收的放射性中有0.35%与质膜结合。3. 地塞米松在体外也能特异性地与质膜结合。以fmol/mg蛋白质表示，地塞米松与质膜的结合与与可溶性细胞质组分（胞质溶胶）的结合相当。4. 使用针对糖皮质激素受体的多克隆抗体和间接免疫荧光技术，可观察到质膜有强烈的荧光标记，表明质膜上有高浓度的糖皮质激素受体。5. 受体在质膜上的定位对于其与最初作用于细胞膜的因子（有丝分裂原、生长因子）相互作用可能具有潜在重要性，这些因子可在细胞核水平调节随后的细胞增殖和生长。