Bergman B, Haskins K
Barbara Davis Center for Childhood Diabetes, Denver, Colorado 80262.
Diabetes. 1994 Feb;43(2):197-203. doi: 10.2337/diab.43.2.197.
Islet-reactive T-cell clones from NOD mice provide an important approach to the investigation of antigens with relevance to type I diabetes. To identify a source of beta-cell antigen suitable for biochemical studies, we have used two islet-specific, diabetogenic T-cell clones to test beta-tumor cells. beta-tumor cell lines, maintained in continuous culture, were found to lose antigenicity rapidly. However, cells harvested directly from beta-tumors arising spontaneously in the transgenic NOD/Lt-Tg(RIPTag)1Lt mouse proved to be a potent source of beta-cell antigen for the T-cell clones. Subcellular fractionation of beta-tumor cells showed that the T-cell antigen was highly enriched in the beta-granule fraction and that this activity was associated with the granule membrane.
来自非肥胖糖尿病（NOD）小鼠的胰岛反应性T细胞克隆为研究与I型糖尿病相关的抗原提供了重要方法。为了确定适合生化研究的β细胞抗原来源，我们使用了两个胰岛特异性、致糖尿病的T细胞克隆来检测β肿瘤细胞。结果发现，在连续培养中维持的β肿瘤细胞系会迅速丧失抗原性。然而，从转基因NOD/Lt-Tg(RIPTag)1Lt小鼠自发产生的β肿瘤中直接收获的细胞被证明是T细胞克隆的β细胞抗原的有效来源。β肿瘤细胞的亚细胞分级分离表明，T细胞抗原在β颗粒部分高度富集，并且这种活性与颗粒膜相关。
