Polack E, Nahmod V E, Emeric-Sauval E, Bello M, Costas M, Finkielman S, Arzt E
Departamento de Sustancias Vasoactivas, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
J Clin Immunol. 1993 Nov;13(6):445-51. doi: 10.1007/BF00920020.
Interferon-gamma (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) in 14 patients with anorexia nervosa (AN) was significantly lower than in 14 age-matched healthy controls. Follow-up samples in four patients displayed low levels, except in two when they recovered the IFN-gamma production as the hormonal cycles were restored. A large interindividual variation for the lymphocyte proliferative response was observed in 30 AN patients. DNA synthesis of PBMC was normal in 8 patients (27%), significantly increased in 6 (20%) (P < 0.001), and significantly decreased in 16 (53%) (P < 0.001). IFN-gamma inhibition was reversed by culturing a control lymphocyte population with monocytes from patients with AN. This was not observed in cultures of control monocytes and AN lymphocytes. IL-2 receptor (TAC subunit) was assessed and no difference was found in the number of TAC-positive cells between patients and controls. These results point out impaired production of the immunomodulator cytokine IFN-gamma as a major functional defect of AN peripheral lymphocytes.
14例神经性厌食症（AN）患者外周血单核细胞（PBMC）产生的干扰素-γ（IFN-γ）显著低于14例年龄匹配的健康对照者。4例患者的随访样本显示水平较低，但有2例在激素周期恢复时IFN-γ产生恢复。在30例AN患者中观察到淋巴细胞增殖反应存在较大的个体间差异。8例患者（27%）的PBMC的DNA合成正常，6例（20%）显著增加（P<0.001），16例（53%）显著减少（P<0.001）。通过将对照淋巴细胞群体与AN患者的单核细胞一起培养，可逆转IFN-γ抑制。在对照单核细胞与AN淋巴细胞的培养中未观察到这种情况。评估了白细胞介素-2受体（TAC亚基），患者与对照者之间TAC阳性细胞数量未发现差异。这些结果指出免疫调节细胞因子IFN-γ产生受损是AN外周淋巴细胞的主要功能缺陷。
