Bergqvist A, Ljungberg O, Skoog L
Department of Obstetrics and Gynaecology, Malmö General Hospital, Sweden.
Hum Reprod. 1993 Nov;8(11):1915-22. doi: 10.1093/oxfordjournals.humrep.a137960.
The objective of the study was to compare the localization and staining intensity of oestrogen and progesterone receptors in endometrium and endometriotic tissue. Using monoclonal antibodies towards oestrogen and progesterone receptors, analysis was performed in 63 endometriotic samples from 40 women and compared to endometrium obtained simultaneously from 25 of the women. Using a staining index, 'total immuno-staining score', calculated from the staining intensity multiplied by the fraction of positive cells, the receptor content was estimated semiquantitatively. The scores for both oestrogen and progesterone receptors were lower in endometriotic epithelial cells than in endometrial epithelial cells, but the differences reached statistical significance only for the progesterone receptor. No difference was found for stromal cells. There was a significant correlation between oestrogen receptor score in endometriotic tissue and in endometrium, but not for progesterone receptor score. In endometrium and vaginal and peritoneal endometriosis, the progesterone receptor score showed similar values, higher than those in ovarian endometriosis. The data from this large immunohistochemical study support previous results of quantitative steroid receptor analyses, indicating that the regulation of steroid effects, especially those of progesterone, differs between endometriotic and endometrial tissue.
本研究的目的是比较雌激素和孕激素受体在子宫内膜和子宫内膜异位组织中的定位及染色强度。使用针对雌激素和孕激素受体的单克隆抗体，对40名女性的63个子宫内膜异位样本进行分析，并与同时从其中25名女性获取的子宫内膜进行比较。通过由染色强度乘以阳性细胞比例计算得出的染色指数“总免疫染色评分”，对受体含量进行半定量评估。子宫内膜异位上皮细胞中雌激素和孕激素受体的评分均低于子宫内膜上皮细胞，但仅孕激素受体的差异具有统计学意义。间质细胞未发现差异。子宫内膜异位组织和子宫内膜中的雌激素受体评分之间存在显著相关性，但孕激素受体评分不存在相关性。在子宫内膜、阴道和腹膜子宫内膜异位症中，孕激素受体评分显示出相似的值，高于卵巢子宫内膜异位症中的值。这项大型免疫组织化学研究的数据支持了先前类固醇受体定量分析的结果，表明类固醇效应，尤其是孕激素效应的调节在子宫内膜异位组织和子宫内膜组织之间存在差异。
