一种用于测量疫苗中破伤风类毒素活性的酶联免疫吸附测定法的开发及其与小鼠毒素中和试验的比较。
Development of an ELISA for measuring the activity of tetanus toxoid in vaccines and comparison with the toxin neutralization test in mice.
作者信息
Manghi M A, Pasetti M F, Brero M L, Deluchi S, di Paola G, Mathet V, Eriksson P V
机构信息
Instituto de Estudios de la Inmunidad Humoral (CONICET-UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
出版信息
J Immunol Methods. 1994 Jan 12;168(1):17-24. doi: 10.1016/0022-1759(94)90204-6.
An enzyme-linked immunosorbent assay (ELISA) has been developed to measure anti-tetanus toxoid antibody levels in immunized guinea-pig sera as a useful alternative to the currently used toxin neutralization test (TNT) in determining the activity of the tetanus toxoid in vaccines. The ELISA was found to measure antibody levels as low as 5.8 x 10(-5) IU/ml. Furthermore, a comparison of the results from ELISA and TNT involving 132 different commercial vaccines showed a very good correlation (r = 0.94, p < 0.001) between antibody levels measured by both methods. The results suggest that the proposed ELISA is a reliable, simple and economical alternative to the TNT in mice for assessing the activity of tetanus toxoids in vaccines.
已开发出一种酶联免疫吸附测定法（ELISA），用于测量免疫豚鼠血清中的抗破伤风类毒素抗体水平，作为目前用于确定疫苗中破伤风类毒素活性的毒素中和试验（TNT）的一种有用替代方法。发现该ELISA可测量低至5.8×10(-5) IU/ml的抗体水平。此外，对132种不同商业疫苗的ELISA和TNT结果进行比较，结果表明两种方法测得的抗体水平之间具有非常好的相关性（r = 0.94，p < 0.001）。结果表明，所提出的ELISA是一种可靠、简单且经济的替代小鼠TNT的方法，用于评估疫苗中破伤风类毒素的活性。
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