Gruen L C, McKimm-Breschkin J L, Caldwell J B, Nice E C
CSIRO Division of Biomolecular Engineering, Parkville, Victoria, Australia.
J Immunol Methods. 1994 Jan 12;168(1):91-100. doi: 10.1016/0022-1759(94)90213-5.
A recently developed alternative to the more traditional techniques for studying antigen-antibody interactions has been examined. This method involves the use of an optical biosensor employing surface plasmon resonance detection. In this system one of the reactants is immobilized on the sensor surface and other reactants are passed over the sensor surface sequentially at a constant flow rate. Binding phenomena are detected in real time from changes in the angle at which surface plasmon resonance occurs. This is dependent, among other things, on changes in the refractive index (which is directly proportional to the mass) at or near to the sensor surface. Applications of this biosensor technique for comparing the binding of related neuraminidases, purified from escape mutants of influenza virus NWS/G70C/75 (N9), to two antibody Fab fragments, are described. These results were compared with those obtained from ELISA and slot blot assays on the same neuraminidases interacting with the same two monoclonal antibodies. The biosensor method was shown to be highly specific, permitting rapid screening of binding in such antigen-antibody systems.
一种最近开发的、用于研究抗原 - 抗体相互作用的替代传统技术已被研究。该方法涉及使用一种采用表面等离子体共振检测的光学生物传感器。在这个系统中，一种反应物固定在传感器表面，其他反应物以恒定流速依次通过传感器表面。结合现象通过表面等离子体共振发生角度的变化实时检测。这尤其取决于传感器表面或其附近折射率（与质量成正比）的变化。描述了这种生物传感器技术在比较从流感病毒NWS/G70C/75（N9）逃逸突变体中纯化的相关神经氨酸酶与两种抗体Fab片段结合方面的应用。将这些结果与在相同神经氨酸酶与相同两种单克隆抗体相互作用的ELISA和狭缝印迹分析中获得的结果进行了比较。生物传感器方法被证明具有高度特异性，能够在这种抗原 - 抗体系统中快速筛选结合情况。
