Jarjour N N, Calhoun W J
Department of Medicine, University of Wisconsin Medical School, Madison.
J Lab Clin Med. 1994 Jan;123(1):131-6.
Oxygen radicals have been implicated in a variety of disease processes including asthma. In this study we investigated the production of superoxide by airspace cells in 56 patients with asthma as compared with 49 normal controls. We found that with patients with asthma with a forced expiratory vital capacity in the 1st second (FEV1) of less than 80% (n = 13) had higher spontaneous superoxide (SO) production when compared with normal subjects (3.6 +/- 1.0 versus 1.9 +/- 0.2 nmol/5 x 10(5) cells/hour, p < 0.01), whereas those with FEV1 > 80% (n = 40) were similar to normal subjects in superoxide generation (2.1 +/- 0.3 nmol/5 x 10(5) cells/hour). Airspace cells from patients with mild asthma and those with moderate asthma had higher phorbol myristate acetate (PMA)-stimulated SO production when compared with those from normal subjects (8.9 +/- 0.7, 11.1 +/- 2.4, and 6.5 +/- 0.4 nmol/5 x 10(5) cells/hour respectively, p < 0.005, r = -0.35, both comparisons). However, PMA-stimulated SO production was similar in both asthmatic subgroups. Finally, spontaneous generation of SO inversely correlated with FEV1% prediction (r = 0.35, p < 0.01) in the asthma group. We conclude that worsening of airway obstruction in asthma is associated with increased spontaneous generation of SO by airspace leukocytes.
氧自由基与包括哮喘在内的多种疾病过程有关。在本研究中，我们调查了56例哮喘患者与49例正常对照者的肺泡细胞中超氧化物的产生情况。我们发现，第一秒用力呼气肺活量（FEV1）低于80%的哮喘患者（n = 13）与正常受试者相比，其自发超氧化物（SO）产生量更高（3.6±1.0对1.9±0.2 nmol/5×10⁵细胞/小时，p < 0.01），而FEV1>80%的患者（n = 40）在超氧化物生成方面与正常受试者相似（2.1±0.3 nmol/5×10⁵细胞/小时）。与正常受试者相比，轻度哮喘患者和中度哮喘患者的肺泡细胞经佛波酯肉豆蔻酸酯乙酸盐（PMA）刺激后的SO产生量更高（分别为8.9±0.7、11.1±2.4和6.5±0.4 nmol/5×10⁵细胞/小时，p < 0.005，r = -0.35，两组比较）。然而，两个哮喘亚组经PMA刺激后的SO产生量相似。最后，哮喘组中SO的自发产生与FEV1%预测值呈负相关（r = 0.35，p < 0.01）。我们得出结论，哮喘患者气道阻塞的加重与肺泡白细胞自发产生SO的增加有关。
