人淋巴细胞巨噬细胞移动抑制因子的结晶及初步X射线衍射研究。

Crystallization and a preliminary X-ray diffraction study of macrophage migration inhibitory factor from human lymphocytes.

作者信息

Suzuki M, Murata E, Tanaka I, Nishihira J, Sakai M

机构信息

Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan.

出版信息

J Mol Biol. 1994 Jan 21;235(3):1141-3. doi: 10.1006/jmbi.1994.1063.

DOI:10.1006/jmbi.1994.1063

PMID:8289313

Abstract

Two crystal forms of the macrophage migration inhibitory factor (MIF) from human lymphocytes have been obtained by the hanging drop method of vapor diffusion from ammonium sulfate solution. A trigonal crystal form belongs to the space group P3(1)21 (P3(2)21), with unit cell dimensions of a = b = 96.4 A and c = 105.5 A. Assuming that the asymmetric unit contains two or three dimers, the Vm value is calculated as 2.9 or 1.9, respectively. A second crystal form is orthorhombic, space group P2(1)2(1)2(1) with unit cell dimensions a = 68.4 A, b = 68.8 A and c = 86.8 A. The Vm value is 2.1 for two dimers in the asymmetric unit. Both crystals diffract to at least 1.9 A and are suitable for X-ray crystallographic structure determination at a high resolution.

摘要

通过从硫酸铵溶液中采用悬滴气相扩散法，已获得了来自人淋巴细胞的巨噬细胞迁移抑制因子（MIF）的两种晶体形式。一种三方晶体形式属于空间群P3(1)21（P3(2)21），晶胞参数为a = b = 96.4 Å，c = 105.5 Å。假设不对称单元包含两个或三个二聚体，则Vm值分别计算为2.9或1.9。第二种晶体形式为正交晶系，空间群P2(1)2(1)2(1)，晶胞参数a = 68.4 Å，b = 68.8 Å，c = 86.8 Å。对于不对称单元中的两个二聚体，Vm值为2.1。两种晶体的衍射分辨率至少为1.9 Å，适用于高分辨率的X射线晶体学结构测定。