Reith W, Ucla C, Barras E, Gaud A, Durand B, Herrero-Sanchez C, Kobr M, Mach B
Jeantet Laboratory of Molecular Genetics, Department of Genetics and Microbiology, University of Geneva Medical School, Centre Médical Universitaire, Switzerland.
Mol Cell Biol. 1994 Feb;14(2):1230-44. doi: 10.1128/mcb.14.2.1230-1244.1994.
RFX1 is a transactivator of human hepatitis B virus enhancer I. We show here that RFX1 belongs to a previously unidentified family of DNA-binding proteins of which we have cloned three members, RFX1, RFX2, and RFX3, from humans and mice. Members of the RFX family constitute the nuclear complexes that have been referred to previously as enhancer factor C, EP, methylation-dependent DNA-binding protein, or rpL30 alpha. RFX proteins share five strongly conserved regions which include the two domains required for DNA binding and dimerization. They have very similar DNA-binding specificities and heterodimerize both in vitro and in vivo. mRNA levels for all three genes, particularly RFX2, are elevated in testis. In other cell lines and tissues, RFX mRNA levels are variable, particularly for RFX2 and RFX3. RFX proteins share several novel features, including new DNA-binding and dimerization motifs and a peculiar dependence on methylated CpG dinucleotides at certain sites.
RFX1是人类乙型肝炎病毒增强子I的反式激活因子。我们在此表明，RFX1属于一个先前未被鉴定的DNA结合蛋白家族，我们已从人和小鼠中克隆出该家族的三个成员，即RFX1、RFX2和RFX3。RFX家族成员构成了先前被称为增强子因子C、EP、甲基化依赖性DNA结合蛋白或rpL30α的核复合物。RFX蛋白共有五个高度保守的区域，其中包括DNA结合和二聚化所需的两个结构域。它们具有非常相似的DNA结合特异性，并且在体外和体内均能形成异源二聚体。所有三个基因的mRNA水平，尤其是RFX2的mRNA水平，在睾丸中升高。在其他细胞系和组织中，RFX mRNA水平各不相同，尤其是RFX2和RFX3。RFX蛋白具有几个新特征，包括新的DNA结合和二聚化基序，以及在某些位点对甲基化CpG二核苷酸的特殊依赖性。
