• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

多个沉默子元件参与调控鸡波形蛋白基因。

Multiple silencer elements are involved in regulating the chicken vimentin gene.

作者信息

Garzon R J, Zehner Z E

机构信息

Department of Biochemistry and Molecular Biophysics, Medical College of Virginia/Virginia Commonwealth University, Richmond 23298.

出版信息

Mol Cell Biol. 1994 Feb;14(2):934-43. doi: 10.1128/mcb.14.2.934-943.1994.

DOI:10.1128/mcb.14.2.934-943.1994
PMID:8289833
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC358448/
Abstract

Vimentin, a member of the intermediate filament protein family, exhibits tissue- as well as development-specific expression. Transcription factors that are involved in expression of the chicken vimentin gene have been described and include a cis-acting silencer element (SE3) that is involved in the down-regulation of this gene (F. X. Farrell, C. M. Sax, and Z. E. Zehner, Mol. Cell. Biol. 10:2349-2358, 1990). In this study, we report the identification of two additional silencer elements (SE1 and SE2). We show by transfection analysis that all three silencer elements are functionally active and that optimal silencing occurs when multiple (at least two) silencer elements are present. In addition, the previously identified SE3 can be divided into three subregions, each of which is moderately active alone. By gel mobility shift assays, all three silencer elements plus SE3 subregions bind a protein which by Southwestern (DNA-protein) blot analysis is identical in molecular mass (approximately 95 kDa). DNase I footprinting experiments indicate that this protein binds to purine-rich sites. Therefore, multiple elements appear to be involved in the negative regulation of the chicken vimentin gene, which may be important in the regulation of other genes as well.

摘要

波形蛋白是中间丝蛋白家族的成员之一，其表达具有组织特异性和发育特异性。已有文献报道了参与鸡波形蛋白基因表达的转录因子，其中包括一个参与该基因下调的顺式作用沉默元件（SE3）（F. X. Farrell、C. M. Sax和Z. E. Zehner，《分子细胞生物学》10:2349 - 2358，1990年）。在本研究中，我们报告了另外两个沉默元件（SE1和SE2）的鉴定。通过转染分析，我们发现所有这三个沉默元件都具有功能活性，并且当存在多个（至少两个）沉默元件时，沉默效果最佳。此外，先前鉴定的SE3可分为三个亚区域，每个亚区域单独作用时活性适中。通过凝胶迁移率变动分析，所有这三个沉默元件以及SE3亚区域都能结合一种蛋白质，经蛋白质免疫印迹（DNA - 蛋白质印迹）分析，该蛋白质的分子量相同（约95 kDa）。DNA酶I足迹实验表明，这种蛋白质与富含嘌呤的位点结合。因此，多个元件似乎参与了鸡波形蛋白基因的负调控，这在其他基因的调控中可能也很重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72bd/358448/9d4f61c18f8c/molcellb00002-0082-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72bd/358448/0f8930587fc7/molcellb00002-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72bd/358448/0be2971c4d17/molcellb00002-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72bd/358448/9d4f61c18f8c/molcellb00002-0082-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72bd/358448/0f8930587fc7/molcellb00002-0081-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72bd/358448/0be2971c4d17/molcellb00002-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72bd/358448/9d4f61c18f8c/molcellb00002-0082-b.jpg

相似文献

1
Multiple silencer elements are involved in regulating the chicken vimentin gene.多个沉默子元件参与调控鸡波形蛋白基因。
Mol Cell Biol. 1994 Feb;14(2):934-43. doi: 10.1128/mcb.14.2.934-943.1994.
2
Identification of a cis-acting DNA antisilencer element which modulates vimentin gene expression.一种调节波形蛋白基因表达的顺式作用DNA抗沉默元件的鉴定。
Mol Cell Biol. 1992 May;12(5):2230-40. doi: 10.1128/mcb.12.5.2230-2240.1992.
3
Two homologous enhancer elements in the chicken vimentin gene may bind a nuclear factor in common with a nearby silencer element.
J Biol Chem. 1995 Oct 27;270(43):25785-91. doi: 10.1074/jbc.270.43.25785.
4
A negative element involved in vimentin gene expression.波形蛋白基因表达中涉及的一个负性元件。
Mol Cell Biol. 1990 May;10(5):2349-58. doi: 10.1128/mcb.10.5.2349-2358.1990.
5
The zinc finger repressor, ZBP-89, binds to the silencer element of the human vimentin gene and complexes with the transcriptional activator, Sp1.锌指阻遏蛋白ZBP-89与人类波形蛋白基因的沉默元件结合，并与转录激活因子Sp1形成复合物。
J Biol Chem. 2000 Apr 28;275(17):12879-88. doi: 10.1074/jbc.275.17.12879.
6
An antisilencer element is involved in the transcriptional regulation of the human vimentin gene.
Gene. 1999 Apr 1;230(1):111-20. doi: 10.1016/s0378-1119(99)00046-3.
7
Identification of two silencers flanking an AP-1 enhancer in the vimentin promoter.
Gene. 1992 Dec 15;122(2):337-43. doi: 10.1016/0378-1119(92)90223-c.
8
cis-Acting elements and transcription factors involved in the intestinal specific expression of the rat calbindin-D9K gene: binding of the intestine-specific transcription factor Cdx-2 to the TATA box.参与大鼠钙结合蛋白-D9K基因肠道特异性表达的顺式作用元件和转录因子：肠道特异性转录因子Cdx-2与TATA盒的结合
Eur J Biochem. 1996 Mar 15;236(3):778-88. doi: 10.1111/j.1432-1033.1996.00778.x.
9
Identification of the 5' regulatory elements of avian lipoprotein lipase gene: synergistic effect of multiple factors.禽脂蛋白脂肪酶基因5'调控元件的鉴定：多种因子的协同作用
Biochim Biophys Acta. 1993 Dec 14;1216(3):375-84. doi: 10.1016/0167-4781(93)90004-w.
10
A 28-bp negative element with multiple factor-binding activity controls expression of the vimentin-encoding gene.一个具有多种因子结合活性的28碱基对负调控元件控制波形蛋白编码基因的表达。
Gene. 1996 Feb 12;168(2):261-6. doi: 10.1016/0378-1119(95)00789-x.

引用本文的文献

1
Development of a Novel Nonradioisotopic Assay and Cdc25B Overexpression Cell Lines for Use in Screening for Cdc25B Inhibitors.用于筛选Cdc25B抑制剂的新型非放射性检测方法及Cdc25B过表达细胞系的开发
Yonsei Med J. 2018 Oct;59(8):995-1003. doi: 10.3349/ymj.2018.59.8.995.
2
Transforming growth factor-β1 induces expression of human coagulation factor XII via Smad3 and JNK signaling pathways in human lung fibroblasts.转化生长因子-β1 通过 Smad3 和 JNK 信号通路诱导人肺成纤维细胞表达人凝血因子 XII。
J Biol Chem. 2010 Apr 9;285(15):11638-51. doi: 10.1074/jbc.M109.045963. Epub 2010 Feb 8.
3
The transcriptional repressor ZBP-89 and the lack of Sp1/Sp3, c-Jun and Stat3 are important for the down-regulation of the vimentin gene during C2C12 myogenesis.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Interaction between an acidic activator and transcription factor TFIIB is required for transcriptional activation.转录激活需要酸性激活剂与转录因子TFIIB之间的相互作用。
Nature. 1993 Jun 24;363(6431):741-4. doi: 10.1038/363741a0.
3
Identification of a negative element in the human vimentin promoter: modulation by the human T-cell leukemia virus type I Tax protein.人波形蛋白启动子中负调控元件的鉴定：人I型T细胞白血病病毒Tax蛋白的调节作用
转录抑制因子ZBP-89以及Sp1/Sp3、c-Jun和Stat3的缺失对于C2C12成肌过程中波形蛋白基因的下调很重要。
Differentiation. 2009 Jun;77(5):492-504. doi: 10.1016/j.diff.2008.12.005. Epub 2009 Feb 23.
4
Promoter-independent regulation of vimentin expression in mammary epithelial cells by val(12)ras and TGFbeta.val(12)ras和TGFβ对乳腺上皮细胞波形蛋白表达的启动子非依赖性调控
Exp Cell Res. 2007 Oct 15;313(17):3718-28. doi: 10.1016/j.yexcr.2007.07.026. Epub 2007 Aug 1.
5
ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1.ZBP-89通过与转录激活因子Sp1相互作用来抑制波形蛋白基因的转录。
Nucleic Acids Res. 2003 Jun 1;31(11):2900-14. doi: 10.1093/nar/gkg380.
6
Tissue-restricted expression of the cardiac alpha-myosin heavy chain gene is controlled by a downstream repressor element containing a palindrome of two ets-binding sites.心脏α-肌球蛋白重链基因的组织特异性表达受一个下游抑制元件控制，该元件包含两个ets结合位点的回文结构。
Mol Cell Biol. 1998 Dec;18(12):7243-58. doi: 10.1128/MCB.18.12.7243.
7
Differential expression of lacZ in the liver and kidney of transgenic mice carrying chimeric lacZ-erythropoietin gene constructs with or without its 1.2 kb 3'-flanking sequence.携带嵌合型lacZ-促红细胞生成素基因构建体（含或不含其1.2 kb 3'侧翼序列）的转基因小鼠肝脏和肾脏中lacZ的差异表达。
Nucleic Acids Res. 1996 Sep 15;24(18):3621-8. doi: 10.1093/nar/24.18.3621.
8
Two regulatory elements of similar structure and placed in tandem account for the repressive activity of the first intron of the human apolipoprotein A-II gene.两个结构相似且串联排列的调控元件导致了人类载脂蛋白A-II基因第一个内含子的抑制活性。
Biochem J. 1996 Sep 1;318 ( Pt 2)(Pt 2):547-53. doi: 10.1042/bj3180547.
9
A transcriptional silencer controls the developmental expression of the CD4 gene.一种转录沉默子控制着CD4基因的发育表达。
EMBO J. 1994 Aug 1;13(15):3570-9. doi: 10.1002/j.1460-2075.1994.tb06664.x.
10
Identification of a cell-type-specific transcriptional repressor in the promoter region of the mouse hepatocyte growth factor gene.
Mol Cell Biol. 1994 Nov;14(11):7046-58. doi: 10.1128/mcb.14.11.7046-7058.1994.
Mol Cell Biol. 1993 Jan;13(1):89-97. doi: 10.1128/mcb.13.1.89-97.1993.
4
DNA topoisomerase I is involved in both repression and activation of transcription.DNA拓扑异构酶I参与转录的抑制和激活过程。
Nature. 1993 Sep 16;365(6443):227-32. doi: 10.1038/365227a0.
5
Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.在哺乳动物细胞中表达氯霉素乙酰转移酶的重组基因组。
Mol Cell Biol. 1982 Sep;2(9):1044-51. doi: 10.1128/mcb.2.9.1044-1051.1982.
6
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89. doi: 10.1093/nar/11.5.1475.
7
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
8
Role of DNA replication in the repression of silent mating type loci in yeast.DNA复制在酵母沉默交配型基因座抑制中的作用。
Nature. 1984;312(5991):247-51. doi: 10.1038/312247a0.
9
pEMBL: a new family of single stranded plasmids.pEMBL：一类新型单链质粒。
Nucleic Acids Res. 1983 Mar 25;11(6):1645-55. doi: 10.1093/nar/11.6.1645.
10
Intermediate filaments: a chemically heterogeneous, developmentally regulated class of proteins.中间丝：一类化学性质各异、受发育调控的蛋白质。
Annu Rev Biochem. 1982;51:219-50. doi: 10.1146/annurev.bi.51.070182.001251.