Primary Industry Development, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, Kensington, Western Australia, 6151, Australia.
Ecosciences Precinct, Queensland Department of Agriculture and Fisheries, GPO Box 46, Brisbane, Queensland, 4001, Australia.
Arch Virol. 2024 Oct 8;169(11):216. doi: 10.1007/s00705-024-06142-z.
Soybean dwarf virus (SbDV; family Tombusviridae, genus Luteovirus, species Luteovirus glycinis) is an RNA plant virus that is transmitted solely by aphids in a persistent, circulative and non-propagative manner. SbDV causes significant losses in cultivated Fabaceae, especially in subterranean clover (Trifolium subterraneum) pastures of mainland Australia. SbDV isolates are classified into four phenotypically distinguishable strains: YP, YS, DP, and DS. Y and D strains differ primarily in their host range, and P and S strains in their primary vector species. Genetically, Y and D strains separate into two clades in every genomic region except for the N-terminal region of the readthrough domain (N-RTD), in which P and S strains separate. SbDV diversity in Australia has yet to be investigated, so in this study, 41 isolates were collected from six different host species across two production regions of Australia: the south coast of Western Australia ('south-west') and northern New South Wales/southern Queensland ('north-east'). A near-complete genome sequence of each isolate was obtained, and together with all 50 whole-genome sequences available in the GenBank database, underwent phylogenetic analysis of the whole genome nt and the N-RTD aa sequences. At the whole-genome level, the isolates separated into D and Y clades. At the N-RTD level, most of the isolates separated into P and S clades. All south-west isolates and 11 of the 31 north-east isolates were in the Y clade, and the remaining 20 north-east isolates were in the D clade. Except for one isolate that fell outside the P and S clades, all south-west and north-east isolates were in the P clade, suggesting that they are transmitted by Acyrthosiphon pisum and Myzus persicae. Available biological data largely supported the phenotypic inferences made from the phylogenetic analysis, suggesting that genetic data can provide critical epidemiological insights, provided that sufficient biological data have been collected.
大豆矮化病毒（SbDV；Tombusviridae 科，Luteovirus 属，Luteovirus glycinis 种）是一种 RNA 植物病毒，仅通过蚜虫以持久、循环和非增殖的方式传播。SbDV 会导致栽培豆科植物，特别是澳大利亚大陆地下三叶草（Trifolium subterraneum）牧场遭受重大损失。SbDV 分离株分为四个表型上可区分的株系：YP、YS、DP 和 DS。Y 株系和 D 株系主要在宿主范围上有所不同，P 株系和 S 株系主要在其初侵染媒介物种上有所不同。从遗传角度来看，除通读域（N-RTD）的 N 端区域外，Y 株系和 D 株系在每个基因组区域都分为两个进化枝，而 P 株系和 S 株系在该区域分离。澳大利亚尚未对 SbDV 多样性进行调查，因此在本研究中，从澳大利亚两个生产区的六个不同宿主物种中收集了 41 个分离株：西澳大利亚州南部海岸（“西南部”）和新南威尔士州北部/昆士兰州南部（“东北部”）。获得了每个分离株的近全长基因组序列，并与 GenBank 数据库中可用的所有 50 个全基因组序列一起，对全基因组 nt 和 N-RTD aa 序列进行了系统发育分析。在全基因组水平上，分离株分为 D 株系和 Y 株系。在 N-RTD 水平上，大多数分离株分为 P 株系和 S 株系。所有西南部分离株和 31 个东北部分离株中的 11 个属于 Y 株系，其余 20 个东北部分离株属于 D 株系。除一个分离株不属于 P 株系和 S 株系外，所有西南部和东北部分离株都属于 P 株系，表明它们是由豌豆蚜和桃蚜传播的。现有的生物学数据在很大程度上支持了从系统发育分析中得出的表型推断，这表明遗传数据可以提供关键的流行病学见解，前提是已经收集了足够的生物学数据。
