Tian Bin, Gildow Frederick E, Stone Andrew L, Sherman Diana J, Damsteegt Vernon D, Schneider William L
Department of Plant Pathology, The Pennsylvania State University, University Park, PA 16802, USA.
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA.
Viruses. 2017 Jun 21;9(6):155. doi: 10.3390/v9060155.
Soybean Dwarf Virus (SbDV) is an important plant pathogen, causing economic losses in soybean. In North America, indigenous strains of SbDV mainly infect clover, with occasional outbreaks in soybean. To evaluate the risk of a US clover strain of SbDV adapting to other plant hosts, the clover isolate SbDV-MD6 was serially transmitted to pea and soybean by aphid vectors. Sequence analysis of SbDV-MD6 from pea and soybean passages identified 11 non-synonymous mutations in soybean, and six mutations in pea. Increasing virus titers with each sequential transmission indicated that SbDV-MD6 was able to adapt to the plant host. However, aphid transmission efficiency on soybean decreased until the virus was no longer transmissible. Our results clearly demonstrated that the clover strain of SbDV-MD6 is able to adapt to soybean crops. However, mutations that improve replication and/or movement may have trade-off effects resulting in decreased vector transmission.
大豆矮缩病毒（SbDV）是一种重要的植物病原体，会给大豆造成经济损失。在北美，SbDV的本土菌株主要感染三叶草，偶尔也会在大豆中爆发。为了评估美国三叶草菌株SbDV适应其他植物宿主的风险，通过蚜虫载体将三叶草分离株SbDV-MD6连续接种到豌豆和大豆上。对来自豌豆和大豆传代的SbDV-MD6进行序列分析，在大豆中鉴定出11个非同义突变，在豌豆中鉴定出6个突变。随着每次连续传代病毒滴度增加，表明SbDV-MD6能够适应植物宿主。然而，蚜虫在大豆上的传播效率下降，直到病毒不再具有传染性。我们的结果清楚地表明，SbDV-MD6的三叶草菌株能够适应大豆作物。然而，改善复制和/或移动的突变可能会产生权衡效应，导致载体传播减少。
