Kuppner M C, Liversidge J, McKillop-Smith S, Lumsden L, Forrester J V
Department of Ophthalmology, University of Aberdeen, Medical School, Foresterhill.
Curr Eye Res. 1993 Oct;12(10):923-34. doi: 10.3109/02713689309020399.
Samples of iris ciliary body, choroid and retina from normal eyes and from 2 cases of sympathetic ophthalmitis (one acute and one late stage fibrosis) were examined for the expression of the VLA integrins beta 1 and alpha 1-6, and the integrin beta 3, in addition to ICAM-1, VCAM-1, ELAM-1 and CD44 using an APAAP staining technique. The expression of VLA-4, VLA-5, VCAM-1, ICAM-1, and CD44 was significantly increased and ELAM-1 was slightly increased in acute sympathetic ophthalmitis in comparison to fibrotic and normal eyes. VLA-6 was moderately increased in acute and fibrotic cases and VLA-2 VLA-3 and beta 3 were moderately expressed on all tissues examined. The differential expression of molecules known to be involved in lymphocyte activation and adhesion in acute sympathetic ophthalmitis suggests that certain adhesion molecules play a role in the pathogenesis of intraocular inflammation and may be suitable targets for immunotherapy.
采用抗碱性磷酸酶-抗碱性磷酸酶复合物（APAAP）染色技术，检测正常眼以及2例交感性眼炎（1例急性期和1例晚期纤维化期）患者的虹膜睫状体、脉络膜和视网膜样本中VLA整合素β1和α1 - 6、整合素β3，以及细胞间黏附分子-1（ICAM-1）、血管细胞黏附分子-1（VCAM-1）、内皮细胞白细胞黏附分子-1（ELAM-1）和CD44的表达情况。与纤维化期和正常眼相比，急性期交感性眼炎中VLA-4、VLA-5、VCAM-1、ICAM-1和CD44的表达显著增加，ELAM-1略有增加。急性期和纤维化期病例中VLA-6中度增加，VLA-2、VLA-3和β3在所有检测组织中均呈中度表达。已知参与淋巴细胞活化和黏附的分子在急性期交感性眼炎中的差异表达表明，某些黏附分子在眼内炎症发病机制中起作用，可能是免疫治疗的合适靶点。
