Schröder K, Zuber P, Willimsky G, Wagner B, Marahiel M A
Biochemie/Fachbereich Chemie, Philipps-Universität Marburg, Germany.
Gene. 1993 Dec 22;136(1-2):277-80. doi: 10.1016/0378-1119(93)90479-m.
The Bacillus subtilis cold shock (CS)-inducible gene, cspB, encoding the nucleic-acid-binding, major CS protein CspB, is located at about 80 degrees on the B. subtilis genetic map. Using this cspB as a probe, the CspB-encoding genes from two thermophilic bacilli were cloned and characterized. The nucleotide (nt) sequences of the B. caldolyticus and B. stearothermophilus cspB coding regions are 78 and 76% identical to the B. subtilis cspB and the deduced amino acid (aa) sequences revealed 84 and 82% identity, respectively. The cspB genes of the mesophilic B. globigii and the some what psychrotrophic B. globisporus, were amplified by PCR using mixed degenerate oligodeoxyribonts based on the 5' and 3' ends of B. subtilis cspB. The nt sequence comparisons of the resulting cloned PCR fragments revealed 98 to 99% identity to cspB of B. subtilis and 97% aa identity to the CspB protein. The high conservation of CspB within the genus Bacillus and the presence of a related nucleic acid-binding domain within several eukaryotic transcription factors implies an important common biological function that seems to be highly conserved from bacteria to man.
枯草芽孢杆菌冷休克（CS）诱导基因cspB编码核酸结合主要CS蛋白CspB，位于枯草芽孢杆菌遗传图谱约80度处。以该cspB为探针，克隆并鉴定了两种嗜热芽孢杆菌中编码CspB的基因。嗜热解淀粉芽孢杆菌和嗜热脂肪芽孢杆菌cspB编码区的核苷酸（nt）序列与枯草芽孢杆菌cspB的序列分别有78%和76%的同一性，推导的氨基酸（aa）序列分别显示出84%和82%的同一性。利用基于枯草芽孢杆菌cspB 5'端和3'端的混合简并寡脱氧核糖核苷酸通过PCR扩增嗜温的球形芽孢杆菌和稍嗜冷的球形孢芽孢杆菌的cspB基因。对所得克隆的PCR片段进行nt序列比较，结果显示与枯草芽孢杆菌cspB有98%至99%的同一性，与CspB蛋白有97%的aa同一性。芽孢杆菌属内CspB的高度保守以及几种真核转录因子中存在相关的核酸结合结构域，意味着存在一种重要的共同生物学功能，该功能似乎从细菌到人类都高度保守。
