Noback M A, Terpstra P, Holsappel S, Venema G, Bron S
Department of Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands.
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3021-6. doi: 10.1099/13500872-142-11-3021.
A 21808 bp nucleotide sequence at 75 degrees on the genetic map of the Bacillus subtilis chromosome was determined. The sequence of this region is adjacent to the glpPFKD operon involved in glycerol utilization. Twenty-six ORFs were identified, one of which corresponds to the cspB gene, encoding a cold-shock protein. Seventeen of the deduced protein sequences of these ORFs displayed significant homology to known proteins in the data banks. One putative operon was identified, consisting of five ORFs, that is probably involved in the uptake and processing of copper. The location of cspB in this sequence does not confirm the genetic mapping data, indicating that the gene is closely linked to comK, which is located at 80 degrees on the B. subtilis chromosome.
测定了枯草芽孢杆菌染色体遗传图谱上75°处一段21808 bp的核苷酸序列。该区域的序列与参与甘油利用的glpPFKD操纵子相邻。鉴定出26个开放阅读框（ORF），其中一个对应于编码冷休克蛋白的cspB基因。这些ORF推导的蛋白质序列中有17个与数据库中的已知蛋白质具有显著同源性。鉴定出一个由五个ORF组成的假定操纵子，可能参与铜的摄取和加工。cspB在该序列中的位置与遗传图谱数据不符，表明该基因与位于枯草芽孢杆菌染色体80°处的comK紧密连锁。
