Gerlach J C, Brombacher J, Courtney J M, Neuhaus P
Chirurgische Klinik, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, Germany.
Int J Artif Organs. 1993 Sep;16(9):677-81.
A comparison of nonenzymatic and enzymatic hepatocyte isolation was performed on pig livers. The collagenase perfusion showed superior results: mean viability 72 +/- 10% versus a maximum viability of 21% using EDTA-perfusion. A five-step collagenase perfusion technique, developed for pig livers enables larger scale investigations, in order to develop methods for hepatocyte cultures in therapeutical liver cell perfusion systems.
对猪肝进行了非酶法和酶法分离肝细胞的比较。胶原酶灌注显示出更好的结果：平均活力为72±10%，而使用乙二胺四乙酸（EDTA）灌注时最大活力为21%。为猪肝开发的五步胶原酶灌注技术能够进行更大规模的研究，以便开发治疗性肝细胞灌注系统中肝细胞培养的方法。
