Chen C L, Wen W N, Chen J Y, Hsu M M, Hsu H C
Department of Pathology, College of Medicine, National Taiwan University, Taipei, Republic of China.
Intervirology. 1993;36(2):91-8. doi: 10.1159/000150327.
To elucidate the possible role of Epstein-Barr virus (EBV) in nasopharyngeal carcinoma (NPC), we investigated the EBV genome in NPC by in situ DNA hybridization using radioisotope- and and biotin-labeled EBV DNA probes. The EBV genome was detected in the tumor cells in all (100%) 60 cases, irrespective of histological type, but not in the lymphocytes. Silver grains, which reflected the copy number of the EBV genome, were more abundant in the nonkeratinizing, spindle cell, and undifferentiated carcinomas than in keratinizing squamous cell carcinoma. In the keratinizing carcinoma, which was poorly differentiated in this series, the EBV genome was usually detected in anaplastic tumor cells, and not in the keratinizing areas. The sensitivity of the radioisotopic technique was superior to that of the biotinylated probe method (100 vs. 81.7%, p < 0.0003). These results suggest that EBV is etiologically related to nasopharyngeal carcinogenesis and that the differentiation of tumor cells in vivo, and probably also in vitro, may become incompatible with EBV replication.
为阐明爱泼斯坦-巴尔病毒（EBV）在鼻咽癌（NPC）中的可能作用，我们使用放射性同位素标记和生物素标记的EBV DNA探针，通过原位DNA杂交研究了NPC中的EBV基因组。在所有60例（100%）病例的肿瘤细胞中均检测到EBV基因组，无论组织学类型如何，但在淋巴细胞中未检测到。反映EBV基因组拷贝数的银颗粒在非角化、梭形细胞和未分化癌中比在角化鳞状细胞癌中更为丰富。在本系列中分化较差的角化癌中，EBV基因组通常在间变肿瘤细胞中检测到，而不在角化区域中检测到。放射性同位素技术的敏感性优于生物素化探针法（100%对81.7%，p<0.0003）。这些结果表明，EBV在病因学上与鼻咽癌发生相关，并且体内肿瘤细胞的分化，可能在体外也是如此，可能与EBV复制不相容。
