Heidenreich F, Leifeld L, Jovin T
Neurologische Klinik, Medizinische Einrichtungen, Heinrich-Heine Universität, Düsseldorf, Germany.
J Neuroimmunol. 1994 Jan;49(1-2):97-108. doi: 10.1016/0165-5728(94)90185-6.
Synthesis of anti-ganglioside GM1 antibodies of the IgM class by peripheral blood mononuclear cells (PBMNC) from patients with immune-mediated neuropathies and motor neuron diseases and from normal controls was stimulated by Pokeweed mitogen (PWM) in vitro. In patients with acute Guillain-Barré syndrome or multifocal motor neuropathy and high serum titers of IgM anti-ganglioside GM1 antibodies this culture response was greatly enhanced as compared to controls and already detectable in unstimulated cultures. Limiting dilution analysis demonstrated high frequencies of GM1-specific B cells in these patients. Anti-ganglioside GM1 antibodies of the IgG and IgA class were only produced by PBMNC from patients with Guillain-Barré syndrome corresponding to serum titers. In cultures taken at intervals over 6 months in vitro B cell activity for IgM, IgG and IgA anti-ganglioside GM1 antibodies in two Guillain-Barré syndrome patients declined accompanied by clinical improvement and falling serum titers. We conclude that GM1-specific PWM-responsive B cells pre-exist in peripheral blood and respond to T cell-dependent stimulation in Guillain-Barré syndrome and multifocal motor neuropathy.
来自免疫介导的神经病和运动神经元疾病患者以及正常对照的外周血单核细胞（PBMNC）在体外受商陆丝裂原（PWM）刺激后，可合成IgM类抗神经节苷脂GM1抗体。在急性吉兰-巴雷综合征或多灶性运动神经病患者且血清IgM抗神经节苷脂GM1抗体滴度较高时，与对照相比，这种培养反应显著增强，且在未受刺激的培养物中即可检测到。极限稀释分析表明这些患者中GM1特异性B细胞的频率很高。IgG和IgA类抗神经节苷脂GM1抗体仅由与血清滴度相对应的吉兰-巴雷综合征患者的PBMNC产生。在两名吉兰-巴雷综合征患者中，体外培养6个月期间每隔一段时间进行的培养显示，针对IgM、IgG和IgA抗神经节苷脂GM1抗体的B细胞活性下降，同时临床症状改善且血清滴度降低。我们得出结论，GM1特异性PWM反应性B细胞预先存在于外周血中，并在吉兰-巴雷综合征和多灶性运动神经病中对T细胞依赖性刺激产生反应。
