使用高效液相色谱法从牛乳、羊乳和山羊乳中分离酪蛋白的比较研究。

Comparative study of the separation of casein from bovine, ovine and caprine milks using HPLC.

作者信息

Kaminarides S E, Anifantakis E M

机构信息

Agricultural University of Athens, Greece.

出版信息

J Dairy Res. 1993 Nov;60(4):495-504. doi: 10.1017/s0022029900027850.

DOI:10.1017/s0022029900027850

PMID:8294606

Abstract

The separation of cows', sheep's and goats' milk casein components by HPLC on a strong anion-exchange (P.L-SAX 8 mu 1000A) column is described. During HLPC, whole caseins of the three kinds of milk behaved differently from conventional separations. The casein components of the three kinds of milk were well resolved under the chromatographic conditions used. HPLC resolved the kappa-caseins better than did ion-exchange chromatography on DEAE cellulose, and was particularly efficient in the case of goats' milk. Goats' and sheep's milks had almost similar chromatographic profiles but these differed considerably from that of cows' milk. Caseins from the sheep and the goat were also similar in that a shallower NaCl gradient was required for the separation of casein components than for cows' milk.

摘要

描述了通过高效液相色谱法（HPLC）在强阴离子交换柱（P.L-SAX 8μm 1000A）上分离牛奶、羊奶和山羊奶的酪蛋白成分。在高效液相色谱分析过程中，这三种奶的全酪蛋白表现与传统分离方法不同。在所用的色谱条件下，这三种奶的酪蛋白成分得到了很好的分离。HPLC对κ-酪蛋白的分离效果优于在DEAE纤维素上的离子交换色谱法，对山羊奶尤其有效。山羊奶和绵羊奶的色谱图几乎相似，但与牛奶的色谱图有很大差异。绵羊和山羊的酪蛋白也有相似之处，即分离酪蛋白成分所需的氯化钠梯度比牛奶的要浅。