Maurer D, Fiebiger E, Reininger B, Wolff-Winiski B, Jouvin M H, Kilgus O, Kinet J P, Stingl G
Department of Dermatology, University of Vienna Medical School, Austria.
J Exp Med. 1994 Feb 1;179(2):745-50. doi: 10.1084/jem.179.2.745.
Suggestive evidence indicates that immunoglobulin E (IgE)-dependent activation of mononuclear phagocytes plays an important pathogenic role in allergic tissue inflammation. Prevailing opinion holds that low affinity IgE receptors are the relevant IgE-binding structures on monocytes/macrophages and that functional events occurring after cross-linking of membrane-bound IgE on these cells are mediated by these receptors. Here we demonstrate that peripheral blood monocytes can bind monomeric IgE via the high affinity IgE receptor (Fc epsilon RI) and that Fc epsilon RI expression on these cells is upregulated in atopic persons. Further, we demonstrate that, upon monocyte adherence to substrate, bridging of monocyte Fc epsilon RI is followed by cell activation. We propose that direct interaction of multivalent allergen with Fc epsilon RI(+)-bound IgE on mononuclear phagocytes results in cell signaling via Fc epsilon RI and that the biological consequences of this event may critically influence the outcome of allergic reactions.
提示性证据表明，免疫球蛋白E（IgE）依赖的单核吞噬细胞激活在过敏性组织炎症中起重要的致病作用。普遍观点认为，低亲和力IgE受体是单核细胞/巨噬细胞上相关的IgE结合结构，并且这些细胞上膜结合IgE交联后发生的功能事件是由这些受体介导的。在此我们证明，外周血单核细胞可通过高亲和力IgE受体（FcεRI）结合单体IgE，并且在特应性个体中这些细胞上的FcεRI表达上调。此外，我们证明，单核细胞黏附于底物后，单核细胞FcεRI的桥接随后会导致细胞激活。我们提出，多价变应原与单核吞噬细胞上FcεRI结合的IgE直接相互作用会通过FcεRI导致细胞信号传导，并且这一事件的生物学后果可能对过敏反应的结果产生关键影响。
