Brière F, Servet-Delprat C, Bridon J M, Saint-Remy J M, Banchereau J
Laboratory for Immunological Research, Schering-Plough, Dardilly, France.
J Exp Med. 1994 Feb 1;179(2):757-62. doi: 10.1084/jem.179.2.757.
During antigen-induced immune responses, human B cells switch isotype from immunoglobulin M (IgM)-IgD to IgG1-4, IgA1-2, or IgE. In the human, no cytokines have yet been demonstrated to act as switch factors for IgG1, IgG2, and IgG3. In this paper, we report that in response to interleukin 10 (IL-10), anti-CD40 activated tonsillar surface IgD+ (sIgD+) B cells are induced to secrete large amounts of IgM, IgG1, and IgG3 but neither IgG2 nor IgG4. Cord blood purified B cells and lymphocytes from Hyper-IgM patients also produced IgG1 and IgG3 after culture with anti-CD40 and IL-10. In contrast, sIgD- isotype-committed B cells produce IgG1, IgG2, and IgG3 when activated through CD40 in the presence of IL-10. Thus, in addition to its growth-promoting and differentiating activities on human B cells, IL-10 may represent a switch factor for IgG1 and IgG3.
在抗原诱导的免疫反应过程中，人类B细胞的免疫球蛋白同种型从免疫球蛋白M（IgM）-IgD转换为IgG1-4、IgA1-2或IgE。在人类中，尚未证实有细胞因子可作为IgG1、IgG2和IgG3的转换因子。在本文中，我们报道，在白细胞介素10（IL-10）作用下，抗CD40激活的扁桃体表面IgD+（sIgD+）B细胞被诱导分泌大量的IgM、IgG1和IgG3，但不分泌IgG2和IgG4。脐血纯化的B细胞以及来自高IgM患者的淋巴细胞在与抗CD40和IL-10共同培养后也产生IgG1和IgG3。相比之下，sIgD-同种型定向的B细胞在IL-10存在的情况下通过CD40激活时会产生IgG1、IgG2和IgG3。因此，除了其对人类B细胞的促生长和分化活性外，IL-10可能代表IgG1和IgG3的转换因子。
