Shimazaki T
Department of Otorhinolaryngology and Head and Neck Surgery, School of Medicine, Kurume University.
Nihon Jibiinkoka Gakkai Kaiho. 1993 Dec;96(12):2044-56. doi: 10.3950/jibiinkoka.96.2044.
The distribution, number, projections and nature of ganglia and ganglionic neurons in the feline larynx were investigated morphologically. Six to eight large oval or spindle-shaped ganglia (including 50-80 ganglionic neurons per ganglion) in rostral portions of the paraglottic spaces, four to six small spindle-shaped ganglions (containing 5-25 ganglionic perikarya in each ganglion) dorsal to the posterior cricoarytenoid muscles and one to three small elliptical ganglia (having 15-25 ganglionic cells in each ganglion) around the inferior laryngeal nerves were observed in the larynx. Each ganglion was covered with a fibrous capsule and ganglionic neurons with a diameter of 25-30 microns totaled 600 to 800. Ganglionic neurons received projections from the dorsal motor nucleus of vagus, the superior cervical ganglion (SCG) and the nodose ganglion (NG) ipsilaterally. On the other hand, ganglionic neurons projected to SCG, NG, ipsilaterally and the laryngeal mucosa bilaterally with ipsilateral predominance. Ganglionic neurons showed acetylcholinesterase positive reactions, presumably parasympathetic. On immunocytochemistry, many vasoactive intestinal polypeptide (VIP)-immunoreactive (ir) neurons, and a few tyrosine hydroxylase (TH)-ir and substance P (SP)-ir cells were recognized in ganglions, but no calcitonin gene-related peptide (CGRP)-ir neurons were found. These VIP, TH, SP-ir neurons did not change after denervation of the ipsilateral superior and recurrent laryngeal nerves. Many VIP- and some TH-, SP-, CGRP-ir fibers were also observed around vessels and glandular cells. The present findings show that intra-laryngeal ganglionic neurons not only have an endogenic cholinergic nature, but are also involved in local sympathetic and sensory nervous systems.
对猫喉内神经节和神经节神经元的分布、数量、投射及性质进行了形态学研究。在声门旁间隙前部观察到6至8个大的椭圆形或纺锤形神经节（每个神经节含50 - 80个神经节神经元），环杓后肌背侧有4至6个小的纺锤形神经节（每个神经节含5 - 25个神经节核周体），喉下神经周围有1至3个小的椭圆形神经节（每个神经节有15 - 25个神经节细胞）。每个神经节被纤维性被膜覆盖，直径为25 - 30微米的神经节神经元总数为600至800个。神经节神经元接受来自同侧迷走神经背运动核、颈上神经节（SCG）和结状神经节（NG）的投射。另一方面，神经节神经元向同侧的SCG、NG投射，并且以同侧为主向双侧喉黏膜投射。神经节神经元显示乙酰胆碱酯酶阳性反应，推测为副交感神经。免疫细胞化学显示，在神经节中可识别出许多血管活性肠肽（VIP）免疫反应性（ir）神经元，以及少数酪氨酸羟化酶（TH）-ir和P物质（SP）-ir细胞，但未发现降钙素基因相关肽（CGRP）-ir神经元。同侧喉上神经和喉返神经去神经支配后，这些VIP、TH、SP-ir神经元无变化。在血管和腺细胞周围也观察到许多VIP-ir纤维以及一些TH-、SP-、CGRP-ir纤维。本研究结果表明，喉内神经节神经元不仅具有内源性胆碱能性质，还参与局部交感和感觉神经系统。
