Rege N N, Dahanukar S A
Dept of Pharmacology, Seth GS Medical College and KEM Hospital, Parel, Bombay, Maharashtra.
J Postgrad Med. 1993 Jan-Mar;39(1):22-5.
An in vitro assay technique was set up to determine the phagocytic and microbicidal activity of a monocyte-macrophage cell line using Candida species as test organisms. The norms were determined for the activity of peritoneal macrophages of rats (24.69 +/- 2.6% phagocytosis and 35.4 +/- 5.22% ICK) and human (27.89 +/- 3.63% phagocytosis and 50.91 +/- 6.3% ICK). The assay technique was used to test the degree of activation of macrophages induced by metronidazole, Tinospora cordifolia and Asparaqus racemousus and to compare their effects with a standard immunomodulator muramyl-dipeptide. All the three test agents increased the phagocytic and killing capacity of macrophages in a dose dependent manner upto a certain dose, beyond which either these activities were found to have plateaued or decreased. The optimal doses for MDP, Metronidazole, Asparagus racemosus and Tinospora cordifolia were found to be 100 micrograms, 300 mg/kg, 200 mg/kg and 100 mg/kg respectively. Patients with cirrhosis were screened for defects in monocyte function. The depressed monocyte function (20.58 +/- 5% phago and 41.24 +/- 12.19% ICK; P < 0.05) was observed indicating a compromised host defense. The utility of this candidicidal assay in experimental and clinical studies is discussed.
建立了一种体外检测技术，以念珠菌属作为测试生物，来测定单核细胞 - 巨噬细胞系的吞噬和杀菌活性。确定了大鼠腹膜巨噬细胞活性的标准（吞噬率为24.69±2.6%，杀菌活性为35.4±5.22%）和人类腹膜巨噬细胞活性的标准（吞噬率为27.89±3.63%，杀菌活性为50.91±6.3%）。该检测技术用于测试甲硝唑、心叶青牛胆和总状天门冬诱导的巨噬细胞活化程度，并将它们的效果与标准免疫调节剂胞壁酰二肽进行比较。所有三种测试药物均以剂量依赖性方式提高巨噬细胞的吞噬和杀伤能力，直至达到一定剂量，超过该剂量后，这些活性要么趋于平稳，要么下降。发现胞壁酰二肽、甲硝唑、总状天门冬和心叶青牛胆的最佳剂量分别为100微克、300毫克/千克、200毫克/千克和100毫克/千克。对肝硬化患者进行单核细胞功能缺陷筛查。观察到单核细胞功能降低（吞噬率为20.58±5%，杀菌活性为41.24±12.19%；P<0.05），表明宿主防御功能受损。讨论了这种杀念珠菌检测在实验和临床研究中的实用性。
