Yoshikawa T, Suzuki H, Sugiyama E, Tunru I S, Ikemoto M, Hori T, Matsui S, Yamashita N, Kobayashi M
Department of Internal Medicine, Toyama Medical and Pharmaceutical University, Sugitani, Japan.
J Rheumatol. 1993 Oct;20(10):1701-6.
To investigate the effects of prostaglandin E1 (PGE1) on IgM and IgG class anti-dsDNA antibody production by young and aged female New Zealand black/white (NZB/W) F1 mouse spleen cells in vitro.
Whole cells or B cells from NZB/W F1 mouse spleen cells were cultured with lipopolysaccharide (LPS) in the absence or presence of graded concentrations of PGE1 for 1 to 5 days. After cultures, the supernatants were collected and assayed for released IgM and IgG class anti-dsDNA antibodies by enzyme-linked immunosorbent assay.
Young (3-month-old) mouse spleen cells produced similar levels of IgM class anti-dsDNA antibodies, while these cells produced considerably low levels of IgG class anti-dsDNA antibodies compared to aged (6-month-old) mouse spleen cells when stimulated with LPS. PGE1 suppressed the production of IgM class anti-dsDNA antibodies by about 50% at a concentration of 10(-6) M in both young and aged mouse spleen cell cultures. On the other hand, the production of IgG class anti-dsDNA antibodies was resistant to the inhibitory effects of PGE1.
Our data suggest that PGE1 is effective in inhibiting the antibody synthesis by B cells precommitted to IgM class anti-dsDNA antibody production, but the production of IgG class anti-dsDNA antibody by memory B cells present in young and aged mice is resistant to the inhibitory effects of PGE1.
研究前列腺素E1（PGE1）对年轻和老年雌性新西兰黑/白（NZB/W）F1小鼠脾细胞体外产生IgM和IgG类抗双链DNA抗体的影响。
将NZB/W F1小鼠脾细胞的全细胞或B细胞与脂多糖（LPS）一起培养，在无或有不同浓度PGE1存在的情况下培养1至5天。培养后，收集上清液，通过酶联免疫吸附测定法检测释放的IgM和IgG类抗双链DNA抗体。
在用LPS刺激时，年轻（3个月大）小鼠脾细胞产生的IgM类抗双链DNA抗体水平相似，而与老年（6个月大）小鼠脾细胞相比，这些细胞产生的IgG类抗双链DNA抗体水平相当低。在年轻和老年小鼠脾细胞培养物中，PGE1在浓度为10^(-6) M时可将IgM类抗双链DNA抗体的产生抑制约50%。另一方面，IgG类抗双链DNA抗体的产生对PGE1的抑制作用具有抗性。
我们的数据表明，PGE1可有效抑制预先倾向于产生IgM类抗双链DNA抗体的B细胞的抗体合成，但年轻和老年小鼠中存在的记忆B细胞产生IgG类抗双链DNA抗体对PGE1的抑制作用具有抗性。
