Sawada S, Talal N
J Immunol. 1979 Jun;122(6):2309-13.
The in vitro production of antibodies to dsDNA was studied with spleen cells from normal and autoimmune mice. After culture for 4 days, the binding of dsDNA in the culture supernatant was measured by a radioimmunoprecipitation assay. The production of antibodies to dsDNA by spleen cells appeared at 15 hr after culture and reached a plateau at 24 hr. No antibodies were produced by thymus cells or splenic T cells. The specificity for dsDNA was shown by competitive inhibition with nonradioactive nucleic acids. Autoimmune strains of mice (NZB/NZW, BXSB, MRL/1) produced more antibodies to dsDNA than did several control strains. Young B/W mice and control strain mice produced mainly IgM antibodies, whereas older B/W mice produced predominantly IgG antibodies to dsDNA. The in vitro production of antibodies to dsDNA by aged B/W spleen cells was macrophage and T cell dependent.
利用正常小鼠和自身免疫小鼠的脾细胞研究了抗双链DNA抗体的体外产生情况。培养4天后，通过放射免疫沉淀试验测定培养上清液中双链DNA的结合情况。脾细胞产生抗双链DNA抗体在培养15小时后出现，并在24小时达到平台期。胸腺细胞或脾T细胞不产生抗体。与非放射性核酸的竞争性抑制显示了对双链DNA的特异性。自身免疫小鼠品系（NZB/NZW、BXSB、MRL/1）产生的抗双链DNA抗体比几个对照品系更多。年轻的B/W小鼠和对照品系小鼠主要产生IgM抗体，而年老的B/W小鼠主要产生抗双链DNA的IgG抗体。老龄B/W脾细胞体外产生抗双链DNA抗体依赖于巨噬细胞和T细胞。
