代谢型谷氨酸受体mGluR5在大鼠脑中的免疫组织化学定位。
Immunohistochemical localization of a metabotropic glutamate receptor, mGluR5, in the rat brain.
作者信息
Shigemoto R, Nomura S, Ohishi H, Sugihara H, Nakanishi S, Mizuno N
机构信息
Department of Morphological Brain Science, Kyoto University, Japan.
出版信息
Neurosci Lett. 1993 Nov 26;163(1):53-7. doi: 10.1016/0304-3940(93)90227-c.
A trpE-fusion protein containing a C-terminal sequence of a rat metabotropic glutamate receptor, mGluR5, was used to produce an antibody. On immunoblot, the antibody specifically reacted with mGluR5 expressed in mammalian cells and rat brain. Immunohistochemical analysis revealed intense mGluR5-like immunoreactivity (LI) in the olfactory bulb, anterior olfactory nuclei, olfactory tubercle, cerebral cortex, hippocampus, lateral septum, striatum, nucleus accumbens, inferior colliculus, and spinal trigeminal nuclei. The distribution pattern of mGluR5-LI corresponds very well with that of mGluR5 mRNA. Electron microscope analysis of the striatum revealed dense accumulation of immunoreaction products in dendrites which were often provided with asymmetrical synapses. These results suggest that mGluR5 is predominantly located in postsynaptic elements.
利用一种包含大鼠代谢型谷氨酸受体mGluR5 C末端序列的trpE融合蛋白制备抗体。在免疫印迹中，该抗体与在哺乳动物细胞和大鼠脑中表达的mGluR5发生特异性反应。免疫组织化学分析显示，在嗅球、前嗅核、嗅结节、大脑皮层、海马体、外侧隔区、纹状体、伏隔核、下丘和三叉神经脊髓核中有强烈的mGluR5样免疫反应性（LI）。mGluR5-LI的分布模式与mGluR5 mRNA的分布模式非常吻合。对纹状体的电子显微镜分析显示，免疫反应产物在树突中密集积累，这些树突通常具有不对称突触。这些结果表明，mGluR5主要位于突触后元件中。
Zotero 插件