Romano C, Sesma M A, McDonald C T, O'Malley K, Van den Pol A N, Olney J W
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St. Louis, MO 63110, USA.
J Comp Neurol. 1995 May 8;355(3):455-69. doi: 10.1002/cne.903550310.
The receptor mGluR5 is a metabotropic glutamate receptor with messenger RNA abundantly present throughout cortex, hippocampus, and caudate/putamen that is also coupled to phosphatidyl inositide hydrolysis and calcium mobilization. In this study, the distribution of mGluR5 was examined in rat brain by immunocytochemistry. The antibody utilized is highly specific and does not cross react with the most closely related other metabotropic glutamate receptor, as determined by Western blot analysis of nonneuronal cells transfected with metabotropic receptor coding sequences. The receptor mGluR5 is widely expressed with the highest density in olfactory bulb, caudate/putamen, lateral septum, cortex, and hippocampus, as confirmed with both immunocytochemistry and Western blot analysis. Electron microscopic studies in hippocampus and cortex indicate that the labeling is mostly on membranes of dendritic spines and shafts. Light and electron microscopic evidence indicates that some mGluR5 immunoreactivity is located in presynaptic axon terminals, suggesting that mGluR5 may function as a presynaptic receptor.
代谢型谷氨酸受体5（mGluR5）是一种代谢型谷氨酸受体，其信使核糖核酸大量存在于整个大脑皮层、海马体以及尾状核/壳核中，并且还与磷脂酰肌醇水解和钙动员相关联。在本研究中，通过免疫细胞化学方法检测了大鼠脑中mGluR5的分布情况。所使用的抗体具有高度特异性，并且根据对转染了代谢型受体编码序列的非神经细胞进行蛋白质免疫印迹分析的结果，该抗体不会与最密切相关的其他代谢型谷氨酸受体发生交叉反应。经免疫细胞化学和蛋白质免疫印迹分析证实，代谢型谷氨酸受体5在嗅球、尾状核/壳核、外侧隔、大脑皮层和海马体中广泛表达，且密度最高。在海马体和大脑皮层进行的电子显微镜研究表明，标记主要位于树突棘和树突轴的膜上。光学显微镜和电子显微镜证据表明，一些mGluR5免疫反应性位于突触前轴突终末，这表明mGluR5可能作为突触前受体发挥作用。
