Bond E F, Heitkemper M M, Jarrett M
Department of Physiological Nursing, University of Washington, Seattle.
Nurs Res. 1994 Jan-Feb;43(1):18-24.
A two-part experimental design was used to study the effects of ovarian hormone cessation, hormone supplementation, and dietary fiber composition on body weight, appetite, and intestinal transit. In Part 1, effects of ovarian hormone status on body weight and baseline and stimulated intestinal transit were measured in chow-fed rats. Sprague-Dawley rats were ovariectomized (OVX), then injected daily (22 days) with estrogen (E), progesterone (P), the combination (E + P), or placebo. Controls were sham operated and placebo injected. Among OVX rats, E and E + P had the least body weight gain (9%, 6%); placebo and P had the greatest (36%, 34%). In OVX-P, baseline intestinal transit (measured in anesthetized rats as distance traveled by a charcoal marker) was relatively low, but vagal stimulation via centrally administered thyrotropin-releasing hormone evoked an increase significantly larger than that in other groups. In Part 2, experiments probed the interacting effects of ovarian hormone cessation and dietary fiber composition on body weight and baseline intestinal transit. Caloric intake was measured to determine the contribution of altered appetite. Rats were OVX or sham operated, then fed liquid diets with or without dietary fiber (25 days). OVX fiber-fed rats had significantly higher caloric intake, weight gain, and baseline intestinal transit than other groups. Caloric intake did not fully account for group differences. These results demonstrate modulation of GI function by ovarian hormones and dietary fiber.
采用两部分实验设计来研究卵巢激素停止、激素补充和膳食纤维组成对体重、食欲及肠道转运的影响。在第一部分中，在喂普通饲料的大鼠中测量卵巢激素状态对体重以及基础和刺激后的肠道转运的影响。将Sprague-Dawley大鼠进行卵巢切除（OVX），然后每天注射（持续22天）雌激素（E）、孕酮（P）、联合用药（E + P）或安慰剂。对照组进行假手术并注射安慰剂。在OVX大鼠中，E组和E + P组体重增加最少（分别为9%、6%）；安慰剂组和P组体重增加最多（分别为36%、34%）。在OVX-P组中，基础肠道转运（在麻醉大鼠中通过木炭标记物行进的距离来测量）相对较低，但通过中枢给予促甲状腺激素释放激素进行迷走神经刺激引起的增加明显大于其他组。在第二部分中，实验探究了卵巢激素停止和膳食纤维组成对体重及基础肠道转运的相互作用。测量热量摄入以确定食欲改变的影响。大鼠进行OVX或假手术，然后喂食含或不含膳食纤维的流质饮食（持续25天）。OVX且喂食纤维的大鼠的热量摄入、体重增加和基础肠道转运显著高于其他组。热量摄入不能完全解释组间差异。这些结果表明卵巢激素和膳食纤维对胃肠功能有调节作用。
