Hrckova G, Halton D W, Maule A G, Brennan G P, Shaw C, Johnston C F
Parasitological Institute, Slovak Academy of Sciences, Kosice.
Parasitol Res. 1993;79(8):690-5. doi: 10.1007/BF00932512.
The distribution pattern and subcellular localisation of neuropeptide F (NPF) immunoreactivity (IR) in the tetrathyridium stage of Mesocestoides corti were investigated by whole-mount immunocytochemistry in conjunction with confocal scanning laser microscopy (CSLM) and by immunoelectron microscopy using immunogold labeling. Using an antiserum directed to the C-terminal decapeptide amide (residues 30-39) of synthetic NPF (Moniezia expansa), CSLM revealed NPF-IR throughout the central and peripheral nervous systems of parental and dividing tetrathyridia. Ultrastructurally, gold labeling of NPF-IR was confined to the contents of the smaller of the two sizes of electron-dense neuronal vesicle identified.
通过整装免疫细胞化学结合共聚焦扫描激光显微镜（CSLM）以及使用免疫金标记的免疫电子显微镜，研究了中殖孔绦虫四槽蚴阶段神经肽F（NPF）免疫反应性（IR）的分布模式和亚细胞定位。使用针对合成NPF（扩展莫尼茨绦虫）C末端十肽酰胺（第30 - 39位氨基酸残基）的抗血清，CSLM显示在亲代和正在分裂的四槽蚴的中枢和外周神经系统中均有NPF-IR。超微结构上，NPF-IR的金标记局限于所鉴定的两种大小的电子致密神经元囊泡中较小的那种囊泡的内容物。
