[Determination of DNA content by means of cytophotometry. Technical and methodological considerations and its application in tumor pathology].

The cytofluorometric determination of ADN content in tumoral cells from 33 tumors is reported. Twenty five epithelial (2 benign and 23 malignant) and 8 non epithelial tumors (1 benign and 7 malignant) were studied. All the benign tumors had a normal ADN content (2c); on the other hand, 65% of malignant tumors had an abnormal ADN content. All non epithelial malignant tumors were aneuploid. Among aneuploid tissues, epithelial tumors were frequently tetraploid (4c) and non epithelial tumors were frequently polyploid 6c, 8c, 12c). It is concluded that this technique gives valuable information about the biological characteristics of tumoral cells and that its results could be incorporated to the routine anatomopathological study of tumoral lesions.