[Stomach and gallbladder cancer. Analysis of cell cycle phases by means of flow cytometry].

The characteristics of the different phases of cell cycle were analyzed in paraffin embedded samples of gall bladder cancer (n = 96) and gastric cancer (n = 40). Computational programs that included Dean, Kosugi and manual methods were used. No differences in variation coefficients were observed between tumors with normal or aneuploid ADN content, with any of the employed methods. Histograms with variation coefficients over 10% were not considered. No differences were observed between the two forms of analysis of Kosugi's method; however there were differences between the manual method and Kosugi's and Dean's methods. The percentage of cells in G0/G1 phase varied between 74.1 and 81.5% according to the employed method. The obtained percentages with Kosugi's method with normal distribution were significantly lower in all the studied groups. These percentages fluctuated between 79.7 and 94.4% in tumors with normal ADN content and between 60.3 and 80.4% in tumors with abnormal ADN content. The proportion of cells in phase G2/M was lower and in phase S lower and more homogeneous among diploid compared to aneuploid tumors. These differences were observed only in primary tumors and not in metastases.