Snounou G, Pinheiro L, Gonçalves A, Fonseca L, Dias F, Brown K N, do Rosario V E
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London, UK.
Trans R Soc Trop Med Hyg. 1993 Nov-Dec;87(6):649-53. doi: 10.1016/0035-9203(93)90274-t.
A method based on the polymerase chain reaction (PCR) for highly sensitive detection and identification of human malaria parasites was applied to blood and mosquito samples obtained from a village in Guinea Bissau. The prevalence of parasites in the human population was shown to be greatly underestimated by microscopical examination. In particular, a high incidence of Plasmodium malariae and P. ovale parasites was revealed only by the PCR assay. Preliminary evidence was obtained to show that the distribution of P. malariae infections within the village was non-random. This was supported by analysis of the parasite species infecting the mosquito vector. The implication of these results for the design and interpretation of epidemiological surveys is discussed.
一种基于聚合酶链反应（PCR）的高灵敏度检测和鉴定人类疟原虫的方法，被应用于从几内亚比绍一个村庄采集的血液和蚊子样本。显微镜检查显示，该村庄人群中寄生虫的流行率被大大低估。特别是，仅通过PCR检测才发现了较高的间日疟原虫和卵形疟原虫感染率。初步证据表明，间日疟原虫感染在村庄内的分布并非随机。感染蚊媒的寄生虫种类分析也支持了这一点。本文讨论了这些结果对流行病学调查设计和解释的意义。
