Fang W, Shi M, Huang L, Shao Q, Chen J
Department of Veterinary Medicine and Animal Science, Zhejiang Agricultural University, Hangzhou, China.
Vet Microbiol. 1993 Oct;37(1-2):115-25. doi: 10.1016/0378-1135(93)90186-b.
The growth of three lactobacilli (Lactobacillus acidophilus, L. bulgaricus and L. casei), Staphylococcus aureus and Escherichia coli was followed in normal and mastitic milk and whey using the standard plate count method. L. acidophilus, L. bulgaricus 6032 and L. casei 6028 grew well in normal milk, but had decreased growth in mastitic milk if not pre-adapted in mastitic whey. S. aureus 26003 and E. coli 44102 showed enhanced growth in mastitic milk as compared with their growth in normal milk. These mastitis pathogens grew faster than the lactobacilli in both the normal milk and the mastitic milk. Among the lactobacilli, L. acidophilus and L. bulgaricus grew faster than L. casei in both types of milk samples. All the bacteria tested grew well in the normal and mastitic whey samples. However, they seemed to have enhanced growth in mastitic whey with the exception of L. casei. Pre-adaptation of the bacteria in mastitic whey in subculture markedly improved the growth of both pathogenic and non-pathogenic bacteria in mastitic milk. All the bacteria showed decreased replication in mastitic milk as compared with mastitic whey.
采用标准平板计数法，对嗜酸乳杆菌、保加利亚乳杆菌、干酪乳杆菌、金黄色葡萄球菌和大肠杆菌在正常乳和患乳房炎乳及乳清中的生长情况进行了跟踪研究。嗜酸乳杆菌、保加利亚乳杆菌6032和干酪乳杆菌6028在正常乳中生长良好，但如果未在患乳房炎乳清中预适应，则在患乳房炎乳中的生长会减少。与在正常乳中的生长相比，金黄色葡萄球菌26003和大肠杆菌44102在患乳房炎乳中生长增强。在正常乳和患乳房炎乳中，这些乳房炎病原体的生长都比乳杆菌快。在两种乳杆菌中，嗜酸乳杆菌和保加利亚乳杆菌在两种类型的乳样品中的生长都比干酪乳杆菌快。所有测试的细菌在正常和患乳房炎乳清样品中生长良好。然而，除干酪乳杆菌外，它们在患乳房炎乳清中的生长似乎有所增强。通过在传代培养中让细菌在患乳房炎乳清中预适应，可显著改善致病性和非致病性细菌在患乳房炎乳中的生长。与患乳房炎乳清相比，所有细菌在患乳房炎乳中的复制均减少。
