Mattila T, O'Boyle D, Frost A J
Department of Veterinary Pathology and Public Health, University of Queensland, Brisbane, Australia.
Microbiol Immunol. 1988;32(7):667-73. doi: 10.1111/j.1348-0421.1988.tb01428.x.
Strains of Staphylococcus aureus producing either diffuse or compact colonies in serum-soft agar were grown in bovine normal and mastitic whey. Bacterial growth was followed by automated turbidometry. Compact strains multiplied faster and to higher final numbers in mastitic whey than diffuse strains, whereas diffuse strains grew to higher numbers in normal whey. Nutrients (hemolysed bovine blood, bovine serum, proteose-peptone) were added to normal whey to enhance bacterial growth as in mastitic whey. The growth-promoting effect of these nutrients was dose-dependent for compact strains but not for diffuse strains. The difference in the growth characteristics of diffuse and compact strains in bovine whey explains some aspects of the pathogenesis of bovine mastitis.
在血清软琼脂中产生弥散或紧密菌落的金黄色葡萄球菌菌株，在牛正常乳清和患乳房炎乳清中培养。通过自动比浊法跟踪细菌生长情况。紧密型菌株在患乳房炎乳清中比弥散型菌株繁殖更快且最终数量更高，而弥散型菌株在正常乳清中生长至更高数量。向正常乳清中添加营养物质（溶血牛血、牛血清、蛋白胨）以促进细菌生长，使其如同在患乳房炎乳清中一样。这些营养物质的促生长作用对紧密型菌株呈剂量依赖性，对弥散型菌株则不然。弥散型和紧密型菌株在牛乳清中生长特性的差异解释了牛乳腺炎发病机制的某些方面。
