Rainard P
Vet Microbiol. 1987 Feb;13(2):159-66. doi: 10.1016/0378-1135(87)90041-1.
The antibacterial activity of milk against a virulent strain of Escherichia coli was investigated using milk fractions from normal or inflamed glands. Mastitic whey exhibited either bactericidal or bacteriostatic activities, depending on whether bacteria were enumerated by the pour plate technique or by surface plating onto sheep blood agar. The former activity was not due to lactoferrin (Lf), which never exerted bactericidal activity, even when assayed in distilled water. Milk whey ultrafiltrate (UF) (mol. wt. less than 5000 d) was used to assay the ability of normal and mastitic milk to support the antibacterial activities of Lf against a strain of E. coli. The addition of purified Lf to UF from mastitic whey resulted in bacteriostasis, whereas Lf was without effect in UF from normal whey. It was concluded that Lf can actually slow down the growth of Lf-sensitive bacteria during mastitis, provided that plasma exudation takes place.
利用来自正常或发炎乳腺的乳汁组分，研究了乳汁对强毒力大肠杆菌菌株的抗菌活性。乳腺炎乳清表现出杀菌或抑菌活性，这取决于细菌是通过倾注平板技术计数还是通过表面接种到绵羊血琼脂平板上计数。前一种活性并非由乳铁蛋白（Lf）引起，即使在蒸馏水中检测，乳铁蛋白也从未发挥过杀菌活性。乳清超滤物（UF）（分子量小于5000 d）用于测定正常乳汁和乳腺炎乳汁支持乳铁蛋白对大肠杆菌菌株抗菌活性的能力。向乳腺炎乳清的超滤物中添加纯化的乳铁蛋白会导致抑菌作用，而乳铁蛋白对正常乳清的超滤物没有影响。得出的结论是，只要发生血浆渗出，乳铁蛋白在乳腺炎期间实际上可以减缓对乳铁蛋白敏感细菌的生长。
