Nakamura Y, Kawazu S, Minami T
First Research Division, National Institute of Animal Health, Tsukuba, Japan.
Vet Microbiol. 1993 Oct;37(1-2):19-30. doi: 10.1016/0378-1135(93)90179-b.
Antigen profiles of Anaplasma ovis and A. mesaeterum were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting, and cross infection trials were performed by A. ovis challenge to A. mesaeterum- or A. marginale-inoculated goats and A. marginale challenge to an A. ovis-inoculated calf. Antigen analysis showed unique and common proteins of A. ovis and A. mesaeterum and identified a major 38 kDa protein to be a dominant immunogen bearing two epitopes common to Anaplasma species. An epitope specific to A. ovis and A. marginale, absent from A. mesaeterum, was recognised on the major protein of A. ovis. Pre-inoculation of goats with A. mesaeterum induced continuous antibody response with low parasitemia and partial protection against A. ovis challenge, as demonstrated by lower peak parasitemia and normal body temperature compared with values for goats inoculated with A. ovis alone. Pre-inoculation of goats with A. marginale and a calf with A. ovis induced weak or no antibody response without parasitemia, and was of no effect for preventing anaplasmosis by heterologous challenge. These results suggest that the development of parasitemia is essential for inducing continuous antibody by which cross protection would be possible.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和免疫印迹分析了绵羊无形体和梅氏无形体的抗原谱，并对接种了梅氏无形体或边缘无形体的山羊进行绵羊无形体攻毒试验，以及对接种了绵羊无形体的犊牛进行边缘无形体攻毒试验。抗原分析显示了绵羊无形体和梅氏无形体独特的和共同的蛋白质，并确定一种主要的38 kDa蛋白质是一种主要免疫原，带有无形体属共有的两个表位。在绵羊无形体的主要蛋白质上识别出了一个绵羊无形体和边缘无形体特有的、梅氏无形体所没有的表位。用梅氏无形体预先接种山羊可诱导持续的抗体反应，寄生虫血症水平较低，并对绵羊无形体攻毒有部分保护作用，与仅接种绵羊无形体的山羊相比，其寄生虫血症峰值较低且体温正常。用边缘无形体预先接种山羊以及用绵羊无形体预先接种犊牛诱导出微弱或无抗体反应且无寄生虫血症，并且对通过异源攻毒预防无形体病没有效果。这些结果表明，寄生虫血症的发展对于诱导持续抗体从而实现交叉保护是必不可少的。
