Blasco J M, Marín C, Jiménez de Bagüés M P, Barberán M
Servicio de Investigación Agraria, Diputación General de Aragón, Zaragoza, Spain.
Vaccine. 1993 Oct;11(13):1291-4. doi: 10.1016/0264-410x(93)90097-h.
The protective efficacy against Brucella ovis of live vaccine Brucella suis strain 2 (S2) and Brucella melitensis strain Rev 1 has been evaluated in rams. Fourteen 4-month-old Brucella-free Aragonesa rams were vaccinated conjunctivally with 2 x 10(9) c.f.u. S2. Sixteen rams of the same breed, condition and age were conjunctivally vaccinated the same day with 1.6 x 10(9) Rev 1. Thirteen rams were unvaccinated controls. Eight months after vaccination all rams were challenged with 6 x 10(9) c.f.u. B. ovis and slaughtered 2 months thereafter for bacteriological and pathological studies. The percentage of infection in the group vaccinated with Rev 1 (43.7%) was significantly lower (p < 0.05) than that of the S2-vaccinated animals (78.6%) and unvaccinated controls (84.6%). No significant differences were found when comparing the percentages of infection corresponding to S2-vaccinated and control groups. The degree of infection (percentage of necropsy samples infected) was significantly lower in Rev 1-vaccinated (13%) than in S2-vaccinated (36.9%) or control groups (47.4%) (p < 0.001). However, no significant differences were found when comparing S2-vaccinated and control groups.
已对公羊评估了猪布鲁氏菌2号菌株（S2）活疫苗和羊布鲁氏菌Rev 1菌株对绵羊布鲁氏菌的保护效力。14只4月龄无布鲁氏菌的阿拉贡公羊通过结膜接种2×10⁹ c.f.u.的S2进行疫苗接种。16只相同品种、状况和年龄的公羊于同一天通过结膜接种1.6×10⁹的Rev 1。13只公羊作为未接种疫苗的对照。接种疫苗8个月后，所有公羊用6×10⁹ c.f.u.的绵羊布鲁氏菌进行攻毒，并在2个月后宰杀以进行细菌学和病理学研究。接种Rev 1的组（43.7%）的感染率显著低于接种S2的动物组（78.6%）和未接种疫苗的对照组（84.6%）（p<0.05）。比较接种S2的组和对照组的感染率时未发现显著差异。接种Rev 1的组（13%）的感染程度（尸检样本感染百分比）显著低于接种S2的组（36.9%）或对照组（47.4%）（p<0.001）。然而，比较接种S2的组和对照组时未发现显著差异。
