Bianchi D W, Yih M C, Zickwolf G K, Flint A F
Division of Genetics, Children's Hospital, Boston, MA.
Am J Obstet Gynecol. 1994 Jan;170(1 Pt 1):202-6. doi: 10.1016/s0002-9378(94)70408-2.
We studied transferrin receptor (CD71) expression in peripheral blood mononuclear cells from healthy pregnant women, to determine if a relationship existed between gestational age and circulating CD71+ mononuclear cells.
Cell suspensions were prepared from venous blood from 139 pregnant women (7 to 26 weeks of gestation), incubated with monoclonal anti-CD71 antibody, and analyzed by flow cytometry.
When only the first sample from each woman was analyzed, extensive biologic variation between women was shown. An apparent biphasic increase in the percentage of CD71+ cells with advancing gestation was suggested. A subgroup of 13 women studied on multiple occasions demonstrated linear increases in CD71+ cells as pregnancy progressed.
Pregnant women, when compared with each other, may have differences in the baseline number of circulating CD71+ cells. The increases seen in individuals studied repeatedly are likely to reflect maternal hematopoiesis and current fetomaternal transfusion.
我们研究了健康孕妇外周血单个核细胞中转铁蛋白受体（CD71）的表达，以确定胎龄与循环CD71+单个核细胞之间是否存在关联。
从139名孕妇（孕7至26周）的静脉血中制备细胞悬液，与单克隆抗CD71抗体孵育，并通过流式细胞术进行分析。
当仅分析每位女性的首个样本时，显示出女性之间存在广泛的生物学差异。提示随着孕周增加，CD71+细胞百分比出现明显的双相增加。对13名多次进行研究的女性亚组进行分析，结果显示随着妊娠进展，CD71+细胞呈线性增加。
相互比较时，孕妇循环CD71+细胞的基线数量可能存在差异。在反复研究的个体中观察到的增加可能反映了母体造血及当前的母胎输血情况。
