Friedmann A S, Malott K A, Memoli V A, Pai S I, Yu X M, North W G
Department of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756.
Br J Cancer. 1994 Feb;69(2):260-3. doi: 10.1038/bjc.1994.49.
Small-cell neuroendocrine carcinoma of the lung is known to express products related to the vasopressin gene, although these products have been reported to sometimes differ from those generated by neurones of the hypothalamo-neurohypophyseal system. To further investigate vasopressin gene expression in neuroendocrine carcinomas, we performed immunohistochemistry on 24 histologically classified small-cell carcinomas using antibodies directed against different regions of the vasopressin precursor. All of the tumours examined contained at least two parts of the vasopressin precursor, suggesting that vasopressin might have a biological role in these tumours and indicating a role for these products in tumour diagnosis and treatment. Sixty-seven per cent of the tumours contained immunoreactivity for all major regions of the precursor: vasopressin, vasopressin-associated human neurophysin, the bridging region between the hormone and the neurophysin, and vasopressin-associated human glycopeptide. However, 33% of the tumours examined appeared to express only part of the vasopressin precursor, as evidenced by the absence of immunoreactivity for the neurophysin and/or the glycopeptide. These results support the proposition that both normal and abnormal vasopressin gene expression occurs in small-cell carcinoma of the lung.
已知肺小细胞神经内分泌癌可表达与血管加压素基因相关的产物，尽管据报道这些产物有时与下丘脑 - 神经垂体系统神经元产生的产物有所不同。为了进一步研究神经内分泌癌中血管加压素基因的表达情况，我们使用针对血管加压素前体不同区域的抗体，对24例经组织学分类的小细胞癌进行了免疫组织化学检测。所有检测的肿瘤均含有血管加压素前体的至少两个部分，这表明血管加压素可能在这些肿瘤中具有生物学作用，并提示这些产物在肿瘤诊断和治疗中具有一定作用。67%的肿瘤对前体的所有主要区域均有免疫反应性：血管加压素、血管加压素相关的人神经垂体素、激素与神经垂体素之间的连接区域以及血管加压素相关的人糖肽。然而，33%的检测肿瘤似乎仅表达血管加压素前体的一部分，这可通过神经垂体素和/或糖肽缺乏免疫反应性得到证明。这些结果支持了肺小细胞癌中存在正常和异常血管加压素基因表达这一观点。
