Guimarães A R, Costa-Rosa L F, Safi D A, Curi R
Departamento de Fisiologia e Biofísica, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 1993 Aug;26(8):813-8.
Diabetic subjects present high susceptibility to infections but the mechanisms involved are not fully known. Macrophages and lymphocytes utilize glucose and glutamine at high rates and these metabolites are important for the function of these cells. The present study examines the activities of key metabolic enzymes in macrophages and lymphocytes obtained from alloxan-diabetic Wistar rats (10 weeks old, 7 rats each group). Since the enteral diet was enriched with omega-6 polyunsaturated fatty acids (PUFA), the effect of these fatty acids was also investigated in the same animals. Diabetes caused a marked decrease of hexokinase activity (48%; 274.23 +/- 18.43 vs 143.29 +/- 10.35 units for control vs diabetic rats) in macrophages and of citrate synthase and glucose-6-phosphate dehydrogenase activities (70%; 321.76 +/- 9.18 vs 96.25 +/- 5.43 units for citrate synthase and 89.43 +/- 2.33 vs 23.13 +/- 1.09 units for G6PDh for control vs diabetic rats) in mesenteric lymph node lymphocytes. A PUFA-rich diet given for 6 weeks enhanced hexokinase activities by 30% (274.23 +/- 18.43 vs 342.48 +/- 15.39, balanced vs PUFA-rich diets for normal and 143.29 +/- 10.35 vs 189.67 +/- 9.57 for diabetic rats) and reduced citrate synthase activities by 43% (30.31 +/- 1.73 vs 17.42 +/- 0.95, balanced vs PUFA-rich diets for normal and 29.34 +/- 1.23 vs 16.73 +/- 1.02 for diabetic rats) in macrophages, and reduced (< 50%; 59.67 +/- 3.45 vs 48.87 +/- 3.37 for hexokinase and 321.76 +/- 2.33 vs 161.66 +/- 9.97 for citrate synthase, balanced vs PUFA-rich diets) the activities of both enzymes in lymphocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
糖尿病患者对感染高度易感，但其中涉及的机制尚不完全清楚。巨噬细胞和淋巴细胞大量利用葡萄糖和谷氨酰胺，这些代谢物对这些细胞的功能很重要。本研究检测了从四氧嘧啶诱导糖尿病的Wistar大鼠（10周龄，每组7只大鼠）获取的巨噬细胞和淋巴细胞中关键代谢酶的活性。由于肠内饮食富含ω-6多不饱和脂肪酸（PUFA），因此也在相同动物中研究了这些脂肪酸的作用。糖尿病导致巨噬细胞中己糖激酶活性显著降低（48%；对照大鼠为274. .23±18.43单位，糖尿病大鼠为143.29±10.35单位），以及肠系膜淋巴结淋巴细胞中柠檬酸合酶和葡萄糖-6-磷酸脱氢酶活性降低（70%；柠檬酸合酶对照大鼠为321.76±9.18单位，糖尿病大鼠为96.25±5.43单位；葡萄糖-6-磷酸脱氢酶对照大鼠为89.43±2.33单位，糖尿病大鼠为23.13±1.09单位）。给予富含PUFA的饮食6周后，巨噬细胞中己糖激酶活性提高了30%（对照大鼠平衡饮食组为274.23±18.43单位，富含PUFA饮食组为342.48±15.39单位；糖尿病大鼠平衡饮食组为143.29±10.35单位，富含PUFA饮食组为189.67±9.57单位），柠檬酸合酶活性降低了43%（对照大鼠平衡饮食组为30.31±1.73单位，富含PUFA饮食组为17.42±0.95单位；糖尿病大鼠平衡饮食组为29.34±1.23单位，富含PUFA饮食组为16.73±1.02单位），并且淋巴细胞中两种酶的活性均降低（<50%；己糖激酶平衡饮食组为59.67±3.45单位，富含PUFA饮食组为48.87±3.37单位；柠檬酸合酶平衡饮食组为321.76±2.33单位，富含PUFA饮食组为161.66±9.97单位）。（摘要截断于250字）
