Otton R, Graziola F, Souza J A, Curi T C, Hirata M H, Curi R
Department of Physiology and Biophysics, University of São Paulo, Butantan, Brazil.
Cell Biochem Funct. 1998 Dec;16(4):253-9. doi: 10.1002/(SICI)1099-0844(1998120)16:4<253::AID-CBF795>3.0.CO;2-#.
The effect of diets enriched with fat containing different fatty acids on glucose and glutamine metabolism of mesenteric lymph nodes lymphocytes, spleen, and thymus and lymphocyte proliferation was examined. The following fat-rich diets were tested: (1) standard chow (CC); (2) medium chain saturated fatty acids (MS)--coconut fat oil; (3) long chain saturated fatty acids (LS)--cocoa butter; (4) monounsaturated fatty acids (MU)--canola oil (n-9); (5) polyunsaturated fatty acids (PU)--soybean oil (n-6). Of the fat-rich diets tested, MS was the one to present the least pronounced effect. Lymphocyte proliferation was reduced by LS (64 per cent), MU (55 per cent), and PU (60 per cent). Hexokinase activity was enhanced in lymph node lymphocytes by PU (67 per cent), in the spleen by MS (42 per cent), and in the thymus by PU (30 per cent). This enzyme activity was reduced in the spleen (33 per cent) by LS and MU (35 per cent). In the thymus, this enzyme activity was reduced by LS (26 per cent) and MU (13 per cent). Maximal phosphate-dependent glutaminase activity was raised in lymphocytes by MS (70 per cent) and MU (20 per cent). This enzyme activity, however, was decreased in lymphocytes by PU (26 per cent), in the spleen by LS (15 per cent), and in the thymus by MU (44 per cent). Citrate synthase activity was increased in lymphocytes by MU (35 per cent), in the spleen by LS (56 per cent) and MU (68 per cent), and in the thymus by LS (42 per cent). This enzyme activity was decreased in lymphocytes by PU (24 per cent) only. [U-14C]-Glucose decarboxylation was raised by all fat-rich diets; MS (88 per cent). LS (39 per cent), MU (33 per cent), and PU (50 per cent), whereas [U-14C]-glutamine decarboxylation was increased by LS (53 per cent) and MU (55 per cent) and decreased by MS (17 per cent). The results presented indicate that the reduction in lymphocyte proliferation due to LS, LU and PU could well be a consequence of changes in glucose and glutamine metabolism.
研究了富含不同脂肪酸的饮食对肠系膜淋巴结淋巴细胞、脾脏、胸腺的葡萄糖和谷氨酰胺代谢以及淋巴细胞增殖的影响。测试了以下富含脂肪的饮食：（1）标准饲料（CC）；（2）中链饱和脂肪酸（MS）——椰子油；（3）长链饱和脂肪酸（LS）——可可脂；（4）单不饱和脂肪酸（MU）——菜籽油（n-9）；（5）多不饱和脂肪酸（PU）——大豆油（n-6）。在所测试的富含脂肪的饮食中，MS的影响最不明显。淋巴细胞增殖受到LS（64%）、MU（55%）和PU（60%）的抑制。PU使淋巴结淋巴细胞中的己糖激酶活性增强（67%），MS使脾脏中的己糖激酶活性增强（42%），PU使胸腺中的己糖激酶活性增强（30%）。LS和MU使脾脏中的该酶活性降低（33%）。在胸腺中，LS（26%）和MU（13%）使该酶活性降低。MS（70%）和MU（20%）使淋巴细胞中最大的磷酸依赖性谷氨酰胺酶活性升高。然而，PU使淋巴细胞中的该酶活性降低（26%），LS使脾脏中的该酶活性降低（15%），MU使胸腺中的该酶活性降低（44%）。MU使淋巴细胞中的柠檬酸合酶活性升高（35%），LS（56%）和MU（68%）使脾脏中的柠檬酸合酶活性升高，LS使胸腺中的柠檬酸合酶活性升高（42%）。仅PU使淋巴细胞中的该酶活性降低（24%）。所有富含脂肪的饮食均使[U-14C]-葡萄糖脱羧增加；MS（88%）、LS（39%）、MU（33%）和PU（50%），而LS（53%）和MU（55%）使[U-14C]-谷氨酰胺脱羧增加，MS使其降低（17%）。所呈现的结果表明，LS、LU和PU导致的淋巴细胞增殖减少很可能是葡萄糖和谷氨酰胺代谢变化的结果。
