Sullivan J T, Weir G O, Brammer S R
Department of Biology, Lamar University, Beaumont, TX 77710.
Dev Comp Immunol. 1993 Nov-Dec;17(6):467-74. doi: 10.1016/s0145-305x(05)80002-3.
Hearts were implanted heterotopically into the hemocoel of NIH albino Biomphalaria glabrata from three xenogeneic donor snails, including two New World (B. obstructa, B. tenagophila) and one Old World (B. alexandrina) species, as well as from wild type allogeneic donors. Recipients were examined histologically at 1, 3, 7, 10, 15, 30, 60, 120, and 180 days postimplantation (PI). Grafts from all four donor species were temporarily infiltrated by recipient hemocytes at early intervals PI and in most instances also became encapsulated. Furthermore, the grafts subsequently showed histopathological alterations and abnormal heartbeat relative to preimplantation controls. However, hemocytic reactions eventually subsided, the implants remained structurally intact, and implant myocardial cells both maintained high levels of intracellular glycogen and continued to contract rhythmically for 6 months. No major differences occurred in fates among different xenografts, or between xenografts and allografts. Our observations of prolonged xenograft survival differ from those of previous investigators, most of whom have reported rapid destruction of implanted xenogeneic tissues in molluscs.
将心脏异位植入来自三只异种供体蜗牛的NIH白化光滑双脐螺的血腔中，这三只供体蜗牛包括两种新大陆（阻隔双脐螺、嗜气管双脐螺）和一种旧大陆（亚历山大双脐螺）的物种，以及野生型同种异体供体。在植入后1、3、7、10、15、30、60、120和180天对受体进行组织学检查。来自所有四种供体物种的移植物在植入后的早期阶段会被受体血细胞暂时浸润，并且在大多数情况下也会被包裹。此外，相对于植入前的对照，移植物随后出现了组织病理学改变和异常心跳。然而，血细胞反应最终消退，植入物在结构上保持完整，植入的心肌细胞既保持了高水平的细胞内糖原，又继续有节奏地收缩了6个月。不同异种移植物之间，或异种移植物与同种异体移植物之间的命运没有出现重大差异。我们对异种移植物长期存活的观察结果与之前研究者的不同，他们中的大多数人报告说，植入软体动物体内的异种组织会迅速被破坏。
