Pham D Q, Hice R H, Sivasubramanian N, Federici B A
Department of Entomology, University of California, Riverside 92521.
Gene. 1993 Dec 31;137(2):275-80. doi: 10.1016/0378-1119(93)90020-4.
A 1629-bp open reading frame (ORF) of Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV) is shown to encode a 78-kDa virion structural protein. To determine this, polyclonal antibody was made to a fusion protein synthesized in Escherichia coli from a chimeric gene that contained 1415 bp of the 1629-bp gene. In Western blot analyses, this antibody cross-reacted with a protein of about 78 kDa in both extracellular virions (ECV) and virions isolated from polyhedra (PDV), and with a 78-kDa protein in PDV envelope preparations, but not with PDV nucleocapsids. This suggests that the protein encoded by the 1629-bp ORF is a virion envelope protein or a protein that occurs in the virion intermediate layer between the envelope and nucleocapsid.
苜蓿银纹夜蛾多核衣壳核型多角体病毒（AcMNPV）的一个1629 bp的开放阅读框（ORF）被证明编码一种78 kDa的病毒粒子结构蛋白。为了确定这一点，针对从包含1629 bp基因中1415 bp的嵌合基因在大肠杆菌中合成的融合蛋白制备了多克隆抗体。在蛋白质印迹分析中，该抗体在细胞外病毒粒子（ECV）和从多角体中分离的病毒粒子（PDV）中均与一种约78 kDa的蛋白质发生交叉反应，并且在PDV包膜制剂中与一种78 kDa的蛋白质发生交叉反应，但与PDV核衣壳不发生交叉反应。这表明由1629 bp ORF编码的蛋白质是一种病毒粒子包膜蛋白或存在于包膜与核衣壳之间的病毒粒子中间层中的蛋白质。
