Asakura T, Adachi K, Shapiro M, Friedman S, Schwartz E
Biochim Biophys Acta. 1975 Dec 15;412(2):197-201. doi: 10.1016/0005-2795(75)90034-3.
Hb Köln (beta 98 Val leads to Met) was found to precipitate rapidly during mechanical shaking. The rate of precipitation of Hb Köln is 5-6 times faster than that of Hb S. The kinetics of precipitation of the patient's hemolysate, which is a mixture of Hb Köln and Hb A, showed a biphasic curve indicating that Hb Köln precipitates independently from Hb A. The instability of Hb Köln may be attributed to the conformational change in the vicinity of heme. The mechanical shaking may be used as a new method for detection and quantitation of hemoglobin Köln and other unstable hemoglobins.
发现科隆血红蛋白（β98缬氨酸突变为甲硫氨酸）在机械振荡过程中迅速沉淀。科隆血红蛋白的沉淀速率比镰状血红蛋白快5至6倍。患者溶血产物（即科隆血红蛋白和A血红蛋白的混合物）的沉淀动力学显示为双相曲线，表明科隆血红蛋白独立于A血红蛋白沉淀。科隆血红蛋白的不稳定性可能归因于血红素附近的构象变化。机械振荡可作为检测和定量科隆血红蛋白及其他不稳定血红蛋白的一种新方法。
