Herndier B, McGrath M, Abbey N, Wang H T, Ng V, Dorigo O, Kaplan L, Müller S, Köhler H
Department of Pathology, San Francisco General Hospital, University of California.
Hybridoma. 1993 Oct;12(5):529-37. doi: 10.1089/hyb.1993.12.529.
The 1F7 idiotype previously defined (J Immunol 147:933;1991 and Eur J Immunol 22:1749;1992) is expressed on antibodies reactive to different proteins of HIV (gp41, p24, gp120). Since serum levels of 1F7 (IgM or IgG) are significantly higher in patients with HIV lymphoma as opposed to HIV-infected individuals, normal controls and non-HIV lymphoma patients, we hypothesized the B-cell neoplasms were the source of the idiotype. However, immunohistochemistry on cryostat sections revealed no 1F7 idiotype signal on neoplastic B-cells nor tumor infiltrative T-cells (n = 8). Interestingly, reactive lymphocytosis adjacent to tumor masses and reactive follicular hyperplastic controls (n = 5) exhibited significant 1F7 reactivity. The reactivity appeared in paracortical and perifollicular lymphoid regions, predominantly regions of B, T and antigen presenting cells in lymph nodes or tonsils. A survey of electrophoretically defined paraproteins with anti-HIV specificities derived from HIV-infected patients showed no Western blot reactivity with the 1F7 anti-idiotypic antibody. Therefore, the idiotype does not appear to be a direct product of B-cell neoplasia or abnormal B-cell proliferation, but is produced by B cell clones responding to HIV infection. This high level of serum 1F7 reactivity could be an important clue in the pathogenesis of HIV lymphomas and confers a highly predictive serological test for HIV lymphoma.
先前定义的1F7独特型（《免疫学杂志》147:933；1991年以及《欧洲免疫学杂志》22:1749；1992年）在与HIV不同蛋白（gp41、p24、gp120）发生反应的抗体上表达。与HIV感染个体、正常对照以及非HIV淋巴瘤患者相比，HIV淋巴瘤患者血清中1F7（IgM或IgG）水平显著更高，因此我们推测B细胞肿瘤是该独特型的来源。然而，对冷冻切片进行免疫组织化学分析显示，在肿瘤性B细胞及肿瘤浸润性T细胞上均未发现1F7独特型信号（n = 8）。有趣的是，肿瘤块旁的反应性淋巴细胞增多以及反应性滤泡增生对照（n = 5）表现出显著的1F7反应性。这种反应性出现在副皮质区和滤泡周围淋巴区域，主要是淋巴结或扁桃体中B细胞、T细胞及抗原呈递细胞所在的区域。一项针对来自HIV感染患者的具有抗HIV特异性的电泳定义副蛋白的调查显示，这些副蛋白与1F7抗独特型抗体在蛋白质印迹分析中无反应性。因此，该独特型似乎并非B细胞肿瘤形成或异常B细胞增殖的直接产物，而是由对HIV感染产生反应的B细胞克隆所产生。血清中1F7的这种高反应性可能是HIV淋巴瘤发病机制中的一个重要线索，并为HIV淋巴瘤提供了一种高度预测性的血清学检测方法。
