Shiota M, Kitamura K, Noda M, Kawanishi Y, Hart S, Miller R A, Mori S
Department of Pathology, University of Tokyo, Japan.
Virchows Arch A Pathol Anat Histopathol. 1992;420(3):263-7. doi: 10.1007/BF01600279.
In an investigation of the immunoglobulin idiotypic expression of non-tumour and neoplastic B lymphocytes in situ, fresh-frozen specimens of reactive tonsils, lymph nodes and B-cell malignant lymphomas (B-MLs) from Japanese patients were studied immunohistochemically with 39 different anti-idiotype antibodies. In reactive lymphoid tissues, while idiotype-bearing cells were largely distributed sparsely in follicles and perifollicular areas, some were heavily crowded in particular germinal centres (GCs), suggesting the presence of oligoclonal proliferations of B-cells in GCs. Forty-eight out of 100 B-MLs reacted with anti-idiotype antibodies. This proportion was significantly higher than those reported in Western cases (27-36%), indicating that Japanese B-MLs share public idiotypes much frequently than western cases. The idiotypes demonstrated in these lymphomas, in contrast to those not expressed in any B-ML, were found much commonly in non-tumour lymphocytes, suggesting that such public idiotypes as were common in B-MLs were frequently shared by normal B-lymphocytes.
在一项关于非肿瘤性和肿瘤性B淋巴细胞原位免疫球蛋白独特型表达的研究中，利用39种不同的抗独特型抗体，对来自日本患者的反应性扁桃体、淋巴结和B细胞恶性淋巴瘤（B-MLs）的新鲜冷冻标本进行了免疫组织化学研究。在反应性淋巴组织中，虽然携带独特型的细胞大多稀疏分布于滤泡和滤泡周围区域，但有些细胞在特定的生发中心（GCs）中高度密集，这表明GCs中存在B细胞的寡克隆增殖。100例B-MLs中有48例与抗独特型抗体发生反应。这一比例显著高于西方病例报告的比例（27%-36%），表明日本B-MLs比西方病例更频繁地共享公共独特型。与在任何B-ML中均未表达的独特型相反，这些淋巴瘤中显示的独特型在非肿瘤淋巴细胞中更为常见，这表明B-MLs中常见的此类公共独特型经常为正常B淋巴细胞所共享。
