Sato T, Harada K, Ohta Y, Kobayashi Y
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Japan.
J Bacteriol. 1994 Feb;176(3):935-7. doi: 10.1128/jb.176.3.935-937.1994.
The Bacillus subtilis spoIVCA gene encodes a site-specific recombinase which creates a sigK gene by DNA rearrangement. We have determined the transcription initiation point of the spoIVCA gene and found that (i) the spoIVCA promoter contains sequences which are similar to -10 and -35 regions of promoters recognized by sigma E and (ii) mutation of spoIIGB, which encodes pro-sigma E, blocked the expression of spoIVCA.
枯草芽孢杆菌spoIVCA基因编码一种位点特异性重组酶,该酶通过DNA重排产生sigK基因。我们已经确定了spoIVCA基因的转录起始点,并发现:(i)spoIVCA启动子包含与σE识别的启动子的-10和-35区域相似的序列;(ii)编码前体σE的spoIIGB的突变阻断了spoIVCA的表达。