Compton C C, Hickerson W, Nadire K, Press W
Department of Pathology, Massachusetts General Hospital, Shriners Hospital for Crippled Children, Boston 02114.
J Burn Care Rehabil. 1993 Nov-Dec;14(6):653-62. doi: 10.1097/00004630-199311000-00010.
Cultured epithelial autografts transplanted to homograft dermis were analyzed histopathologically from 9 days to 2.25 years after grafting and compared with cultured epithelial autografts transplanted to wound beds devoid of homograft dermis in the same patient. The results suggested that grafting to a dermal substrate accelerates biologic events in skin regeneration from cultured epithelial autografts. Both rete ridge development and normalization of the epidermal keratin program from a "hyperproliferative" to a normal pattern occurred months sooner in the cultured epithelial autografts placed on a dermal matrix. Regeneration of anchoring fibrils was also accelerated. Neither repopulation by Langerhans cells nor repigmentation appeared to be altered significantly by grafting to dermal tissue. By special stain, the elastin network of the allograft dermis could still be visualized at 2.25 years, indicating long-term persistence of the homograft matrix. By clinical assessment the rate of successful engraftment ("take rate") of cultured epithelial autografts grafted to allodermis was estimated to be about 95%, and long-term durability was excellent.
对移植到同种异体真皮上的培养自体上皮移植片在移植后9天至2.25年进行组织病理学分析，并与移植到同一患者无同种异体真皮的创面床的培养自体上皮移植片进行比较。结果表明，移植到真皮基质上可加速培养自体上皮移植片皮肤再生中的生物学过程。在置于真皮基质上的培养自体上皮移植片中， rete嵴的发育以及表皮角质形成程序从“过度增殖”模式恢复到正常模式都要早几个月发生。锚定原纤维的再生也加快了。移植到真皮组织中，朗格汉斯细胞的重新填充和色素沉着似乎均未受到明显影响。通过特殊染色，在2.25年时仍可观察到同种异体真皮的弹性蛋白网络，表明同种异体基质长期存在。通过临床评估，移植到同种异体真皮上的培养自体上皮移植片的成功植入率（“成活率”）估计约为95%，且长期耐久性良好。
